推荐产品
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen ~50 kDa
种属反应性
rat, human, mouse
浓度
~1.0 mg/mL
技术
immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells
western blot: 0.5-1 μg/mL using PC12 cell lysates
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... YBX1(4904)
mouse ... Ybx1(22608)
rat ... Ybx1(500538)
一般描述
Y-box-结合蛋白（YB-1）也称为核酸酶敏感元件结合蛋白1（NSEP1），是包含冷休克结构域（CSD）的蛋白质的成员。它由三个结构域组成：N末端被认为是反式激活调节结构域，高度保守的核酸结合结构域（CSD）和C末端结构域，参与蛋白质-蛋白质相互作用。
特异性
抗YB-1（C末端）抗体对大鼠、小鼠和人类的YB-1具有特异性。该免疫肽特异性抑制免疫印迹法检测YB-1条带。
免疫原
合成肽对应于与KLH偶联的人YB-1氨基酸307-324。在大鼠和小鼠YB-1蛋白中的相应序列是相同的。
应用
抗YB-1（C末端）抗体已用于
- 免疫印迹
- 免疫沉淀
- 免疫荧光
- 双重免疫染色
生化/生理作用
Y-box结合蛋白（YB-1）显示与Y-box启动子元件结合以调节基因表达。它在多种细胞功能中起着重要作用，例如转录和翻译调节，DNA修复，耐药性和应激反应。
外形
溶于含有15 mM叠氮化钠的0.01M磷酸盐缓冲盐水（pH 7.4）溶液中。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts
Wei W J, et al.
Nucleic Acids Research, 40(17), 8622-8636 (2012)
The Y-box binding protein 1 suppresses Alzheimer?s disease progression in two animal models
Bobkova N V, et al.
PLoS ONE, 10(9), e0138867-e0138867 (2015)
Kimitoshi Kohno et al.
BioEssays : news and reviews in molecular, cellular and developmental biology, 25(7), 691-698 (2003-06-20)
The Y-box-binding protein (YB-1) represents the most evolutionary conserved nucleic-acid-binding protein currently known. YB-1 is a member of the cold-shock domain (CSD) protein superfamily. It performs a wide variety of cellular functions, including transcriptional regulation, translational regulation, DNA repair, drug
N V Bobkova et al.
PloS one, 10(9), e0138867-e0138867 (2015-09-24)
The Y-box binding protein 1 (YB-1) is a member of the family of DNA- and RNA binding proteins. It is involved in a wide variety of DNA/RNA-dependent events including cell proliferation and differentiation, stress response, and malignant cell transformation. Previously
Anja Bernhardt et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(10), e21912-e21912 (2021-09-18)
High salt diet (HSD) is a hallmark of blood pressure elevations, weight gain and diabetes onset in the metabolic syndrome. In kidney, compensatory mechanisms are activated to balance salt turnover and maintain homeostasis. Data on the long-term effects of HSD
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