推荐产品
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~50 kDa
种属反应性
rat, human, mouse
浓度
~1.0 mg/mL
技术
immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells
western blot: 0.5-1 μg/mL using PC12 cell lysates
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... YBX1(4904)
mouse ... Ybx1(22608)
rat ... Ybx1(500538)
一般描述
Y-box-结合蛋白(YB-1)也称为核酸酶敏感元件结合蛋白1(NSEP1),是包含冷休克结构域(CSD)的蛋白质的成员。它由三个结构域组成:N末端被认为是反式激活调节结构域,高度保守的核酸结合结构域(CSD)和C末端结构域,参与蛋白质-蛋白质相互作用。
特异性
抗YB-1(C末端)抗体对大鼠、小鼠和人类的YB-1具有特异性。该免疫肽特异性抑制免疫印迹法检测YB-1条带。
免疫原
合成肽对应于与KLH偶联的人YB-1氨基酸307-324。在大鼠和小鼠YB-1蛋白中的相应序列是相同的。
应用
抗YB-1(C末端)抗体已用于
- 免疫印迹
- 免疫沉淀
- 免疫荧光
- 双重免疫染色
生化/生理作用
Y-box结合蛋白(YB-1)显示与Y-box启动子元件结合以调节基因表达。它在多种细胞功能中起着重要作用,例如转录和翻译调节,DNA修复,耐药性和应激反应。
外形
溶于含有15 mM叠氮化钠的0.01M磷酸盐缓冲盐水(pH 7.4)溶液中。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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储存分类代码
12 - Non Combustible Liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Cells, 13(5) (2024-03-13)
Fibrosis is characterized by excessive extracellular matrix formation in solid organs, disrupting tissue architecture and function. The Y-box binding protein-1 (YB-1) regulates fibrosis-related genes (e.g., Col1a1, Mmp2, and Tgfβ1) and contributes significantly to disease progression. This study aims to identify
The Y-box binding protein 1 suppresses Alzheimer?s disease progression in two animal models
PLoS ONE, 10(9), e0138867-e0138867 (2015)
BioEssays : news and reviews in molecular, cellular and developmental biology, 25(7), 691-698 (2003-06-20)
The Y-box-binding protein (YB-1) represents the most evolutionary conserved nucleic-acid-binding protein currently known. YB-1 is a member of the cold-shock domain (CSD) protein superfamily. It performs a wide variety of cellular functions, including transcriptional regulation, translational regulation, DNA repair, drug
PloS one, 10(9), e0138867-e0138867 (2015-09-24)
The Y-box binding protein 1 (YB-1) is a member of the family of DNA- and RNA binding proteins. It is involved in a wide variety of DNA/RNA-dependent events including cell proliferation and differentiation, stress response, and malignant cell transformation. Previously
YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts
Nucleic Acids Research, 40(17), 8622-8636 (2012)
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