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XNAT2

Sigma-Aldrich

Extract-N-Amp ™ 组织 PCR 试剂盒

sufficient for 100 extractions, sufficient for 100 amplifications

别名:

Tissue direct PCR kit

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About This Item

UNSPSC代码:
41106303
NACRES:
NA.55

用途

sufficient for 100 amplifications
 sufficient for 100 extractions
 sufficient for 100 reactions

质量水平

200

特点

dNTPs included
hotstart

技术

PCR: suitable

颜色

colorless

运输

wet ice

储存温度

−20°C

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相关类别

一般描述

Extract-N-Amp™组织PCR试剂盒为从各种细胞和组织中快速提取DNA并通过直接PCR（聚合酶链反应）扩增靶标提供了所有必需试剂。这种新型提取方法无需长时间的酶消化或均质化处理。该试剂盒还包括专门配制的热启动PCR ReadyMix™，可直接从提取物中扩增。

应用

Extract-N-Amp™组织PCR试剂盒用于以鼠尾活组织提取基因组DNA和聚合酶链式反应（PCR）。

特点和优势

  • 快速 – 在15 分钟内快速提取用于 PCR 的基因组 DNA
  • 方便-不需要漫长的酶消化或柱纯化
  • 实用 – 基因分型用快速基因组 DNA 分离的完美选择
  • 灵活 – 可提供适合鼠尾、毛发、动物组织、唾液和口腔拭子的操作方案
  • 特异性-用于基因组 DNA 高特异性 PCR 扩增的热启动抗体
  • 提取物在4oC下可稳定保存至少 6 个月。

组分

试剂盒含有Tissue Preparation Solution、Extraction Solution和Extract-N-Amp PCR Reaction Mix。

原理

该试剂盒包含从小鼠尾、毛发、动物组织、唾液和口腔拭子中提取和扩增基因组DNA的经验证实验方案。在常规操作步骤中，基因组DNA提取自在室温下的组织制备溶液和提取溶液中孵育10分钟的样本。将该样本加热至95 °C并持续3分钟，然后混入第三种溶液以便在PCR反应前中和抑制物。随后将一部分DNA提取物加入到含有引物和Extract-N-Amp PCR ReadyMix（包含在试剂盒内）的PCR反应物中。

其他说明

更多信息，请见www.sigma-aldrich.com/extract-n-amp。

法律信息

凡购买本产品，即表示授权用户使用产品说明书所指定的专利，但仅限于将所购数量的产品用于内部研究用途。我们未明确表示、暗示，或通过禁止反言形式转让任何其他专利权（如，5′ Nuclease Process专利权）。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

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警示用语：

Danger

危险分类

Aquatic Chronic 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

靶器官

Respiratory system

储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品
含少量动物源组分生物产品

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Bhaswati Bhattacharya et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(25), 14280-14291 (2020-06-10)
In utero mammalian development relies on the establishment of the maternal-fetal exchange interface, which ensures transportation of nutrients and gases between the mother and the fetus. This exchange interface is established via development of multinucleated syncytiotrophoblast cells (SynTs) during placentation.
Xinyuan Ma et al.
Parasites & vectors, 13(1), 321-321 (2020-06-24)
The plant-parasitic nematode Hoplolaimus columbus is a pathogen that uses a wide range of hosts and causes substantial yield loss in agricultural fields in North America. This study describes, for the first time, the complete mitochondrial genome of H. columbus
James A Green et al.
BMC neuroscience, 14, 96-96 (2013-09-10)
Myelinated axons are organized into distinct subcellular and molecular regions. Without proper organization, electrical nerve conduction is delayed, resulting in detrimental physiological outcomes. One such region is the paranode where axo-glial septate junctions act as a molecular fence to separate
Gergely Vida et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 25(12), 4476-4485 (2011-08-16)
The nervous system is classically organized into sympathetic and parasympathetic systems acting in opposition to maintain physiological homeostasis. Here, we report that both systems converge in the activation of β2-adrenoceptors of splenic regulatory lymphocytes to control systemic inflammation. Vagus nerve
Chelsea D Mullins et al.
PloS one, 8(6), e67941-e67941 (2013-07-05)
Activating mutations in Ras (N- and K-) are the most common point mutations found in patients with multiple myeloma (MM) and are associated with poor clinical outcome. We sought to directly examine the role of Ras activation in MM pathogenesis
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