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用途
sufficient for 100 extractions
sufficient for 500 amplifications
sufficient for 500 reactions
质量水平
特点
dNTPs included
hotstart
技术
PCR: suitable
颜色
colorless
运输
wet ice
储存温度
−20°C
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一般描述
Extract-N-Amp 植物PCR试剂盒含有从植物叶片中快速提取基因组DNA并通过PCR反应扩增感兴趣的目标基因必需的所有试剂。使用新型Extraction Solution，无需通过液氮对植物组织进行传统的冰冻，机械式破碎、有机萃取或沉淀DNA操作。该试剂盒同时包含一种专为从提取物直接扩增而配制的PCR ReadyMix™。
基因组DNA提取自0.5至0.7 cm的植物叶盘，该叶盘通过标准打孔器获得并在95 oC下Extraction Solution中孵育10分钟。向提取物中加入等体积的Dilution Solution，以便在PCR反应前中和抑制物。随后将一部分DNA提取物加入到含有引物和Extract-N-Amp PCR ReadyMix（包含在试剂盒内）的PCR反应物中。
基因组DNA提取自0.5至0.7 cm的植物叶盘，该叶盘通过标准打孔器获得并在95 oC下Extraction Solution中孵育10分钟。向提取物中加入等体积的Dilution Solution，以便在PCR反应前中和抑制物。随后将一部分DNA提取物加入到含有引物和Extract-N-Amp PCR ReadyMix（包含在试剂盒内）的PCR反应物中。
Extract-N-Amp™植物PCR试剂盒用于直接PCR（聚合酶链反应）。该试剂盒提供了从植物叶片快速提取基因组DNA以及通过PCR扩增靶标所需的所有试剂。它是一种创新的提取解决方案，无需常规的植物组织冷冻处理。
The PCR reaction mix facilitates amplification directly from the extract by using an antibody-based hot-start DNA polymerase for specific amplification.
应用
Extract-N-Amp™植物PCR试剂盒适用于基因分型。
它已被用于从新鲜蘑菇中提取DNA。
它已被用于从新鲜蘑菇中提取DNA。
Extract-N-Amp™ Plant PCR Kit has been used to extract plant genomic DNA and in polymerase chain reaction (PCR).
特点和优势
- 可在15分钟内一步提取出用于PCR反应的植物基因组DNA
- 专为直接从提取物进行扩增而配置的PCR ReadyMix™
- 适用于基因组DNA的高度特异性PCR扩增的热启动抗体
- 提取物可在4 °C 下至少稳定保存6个月
- PCR用植物基因组DNA可以在15分钟内提取。
- 无需冷冻、机械破碎、有机溶剂萃取、柱纯化或沉淀
- 经过特别配制的PCR ReadyMix 和提取的DNA一起使用
- 热启动抗体确保基因组DNA的高度特异性PCR扩增
- 适用于高通量植物基因分析
- 无需长时间的酶消化，节省时间和精力
- 提取的DNA在4 °C 下至少6个月保持稳定。
组分
Extract-N-Amp™植物PCR试剂盒包括稀释溶液、提取溶液和Extract-N-Amp PCR 反应混合物（2X）（缓冲液、盐、dNTP、Taq聚合酶和JumpStart Taq抗体）。
Extraction Solution- E7526-12 mL
Dilution Solution- D5688-12mL
Extract-N-Amp PCR ReadyMix. This 2X PCR reaction mix contains buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody.- E3004- 5 x 1.2 ml
Collection tubes
Dilution Solution- D5688-12mL
Extract-N-Amp PCR ReadyMix. This 2X PCR reaction mix contains buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody.- E3004- 5 x 1.2 ml
Collection tubes
其他说明
Extract-N-Amp植物PCR试剂盒仅供实验室使用。不可用于药物、家庭或其他用途。
The Extract-N-Amp™ Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
法律信息
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
相关产品
产品编号
说明
价格
储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
S A Weller et al.
Journal of microbiological methods, 70(2), 379-383 (2007-06-26)
Real-time (TaqMan) PCR assays were developed to detect the strawberry angular leaf spot pathogen Xanthomonas fragariae (Xf) and the strawberry bacterial blight pathogen Xanthomonas arboricola pv. fragariae (Xaf). The Xf PCR (Xf gyrB) was designed within regions of the gyraseB
Noa Eliahu et al.
Eukaryotic cell, 6(3), 421-429 (2007-01-24)
The maize pathogen Cochliobolus heterostrophus requires two mitogen-activated protein kinases (MAPKs), Chk1 and Mps1, to produce normal pigmentation. Young colonies of mps1 and chk1 deletion mutants have a white and autolytic appearance, which was partially rescued by a hyperosmotic environment.
Sophie Lev et al.
Molecular plant-microbe interactions : MPMI, 22(9), 1093-1103 (2009-08-07)
Phosphorylated mitogen-activated protein kinases (MAPK) transmit signals by activation of their targets. The extent of signal transduction could depend on MAPK phosphorylation level, concentration, and subcellular localization. The pathogenicity MAPK Chk1 of the fungal corn pathogen Cochliobolus heterostrophus is required
Len N Gillman et al.
Journal of evolutionary biology, 23(6), 1327-1330 (2010-05-12)
A faster rate of nuclear DNA evolution has recently been found for plants occupying warmer low latitudes relative to those in cooler high latitudes. That earlier study by our research group compared substitution rates within the variable internal transcribed spacer
Rena Shimizu et al.
Plant physiology, 149(2), 841-850 (2008-12-17)
The WUSCHEL-related homeobox (WOX) gene PRESSED FLOWER1 (PRS1) performs a conserved function during lateral organ development in Arabidopsis (Arabidopsis thaliana). Expressed in the periphery of the shoot meristem, PRS1 recruits founder cells that form lateral domains of vegetative and floral
商品
Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
简单的DNA和RNA纯法方法大幅推动了基因组和基因表达的分析和鉴定。人们需要快速、方便地从多种细胞来源分离DNA和RNA，包括哺乳动物、植物和细菌培养物来源的细胞和组织。
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
实验方案
The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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