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一般描述
Extract-N-Amp™血液PCR试剂盒提供了从全血快速提取DNA以及通过直接PCR扩增靶标所需的所有试剂。这种新型提取系统无需进行任何类型的纯化、有机提取、离心、加热、过滤或酒精沉淀。该试剂盒还包括专门配制的PCR ReadyMix™，可直接从提取物中扩增。这种配方使用基于抗体的热启动，用于特异性扩增。
应用
Extract-N-Amp™血液PCR试剂盒已用于从基因组和干血斑中提取DNA。还用于聚合酶链式反应（PCR）。
特点和优势
- 高效8分钟预制品允许更大的速度和吞吐量
- 不需要任何类型的净化、有机提取、离心或酒精沉淀
- 简单的3步程序，无需特殊设备
- 包括Hot start抗体，用于基因组DNA的高特异性PCR扩增
- 兼容多种格式（单管或96孔板）
- 可与全血或血卡一起使用
- 在4℃稳定提取至少6个月
原理
基因组DNA从10 μl全血中提取，只需加入提取液并在室温下孵育5分钟。PCR前，将中和液添加至提取液以中和抑制物质。然后将一部分DNA提取物添加至专门配制的PCR Mix的PCR ReadyMix中。
其他说明
有关其他信息，请参阅www.sigma-aldrich.com/extract-n-amp。
法律信息
购买本产品包括豁免根据产品说明书中所规定的专利提起的诉讼，仅可将所购买的量用于购买者自己的内部研究。′其他专利权（例如5′核酸酶工艺的专利权）不得明示、暗示或禁止反言。 有关购买许可的更多信息，可联系许可总监（Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA）获取。
根据美国专利号5,338,671和5,587,287以及其他国家的相应专利批准用于体外研究的抗体。
根据美国专利号5,338,671和5,587,287以及其他国家的相应专利批准用于体外研究的抗体。
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
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商品
The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.
Extract-N-Amp simplifies DNA extraction and amplification, enabling PCR directly from FTA blood cards, reducing variability.
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
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