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XNAB2

Sigma-Aldrich

Extract-N-Amp™血液PCR试剂盒

sufficient for 100 extractions, sufficient for 100 amplifications

别名:

血液直接 PCR 试剂盒

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About This Item

UNSPSC代码:
41106303
NACRES:
NA.55

用途

sufficient for 100 amplifications
 sufficient for 100 extractions
 sufficient for 100 reactions

质量水平

200

特点

dNTPs included
hotstart

技术

PCR: suitable

颜色

colorless

运输

wet ice

储存温度

−20°C

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相关类别

一般描述

Extract-N-Amp™血液PCR试剂盒提供了从全血快速提取DNA以及通过直接PCR扩增靶标所需的所有试剂。这种新型提取系统无需进行任何类型的纯化、有机提取、离心、加热、过滤或酒精沉淀。该试剂盒还包括专门配制的PCR ReadyMix™，可直接从提取物中扩增。这种配方使用基于抗体的热启动，用于特异性扩增。

应用

Extract-N-Amp™血液PCR试剂盒已用于从基因组和干血斑中提取DNA。它还用于聚合酶链反应（PCR）。

特点和优势

  • 高效8分钟预制品允许更大的速度和吞吐量
  • 不需要任何类型的净化、有机提取、离心或酒精沉淀
  • 简单的3步程序，无需特殊设备
  • 包括Hot start抗体，用于基因组DNA的高特异性PCR扩增
  • 兼容多种格式（单管或96孔板）
  • 可与全血或血卡一起使用
  • 在4℃稳定提取至少6个月

原理

基因组DNA从10 μl全血中提取，只需加入提取液并在室温下孵育5分钟。PCR前，将中和液添加至提取液以中和抑制物质。然后将一部分DNA提取物添加至专门配制的PCR Mix的PCR ReadyMix中。

其他说明

有关其他信息，请参阅www.sigma-aldrich.com/extract-n-amp。

法律信息

购买本产品包括豁免根据产品说明书中所规定的专利提起的诉讼，仅可将所购买的量用于购买者自己的内部研究。′其他专利权（例如5′核酸酶工艺的专利权）不得明示、暗示或禁止反言。 有关购买许可的更多信息，可联系许可总监（Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA）获取。
根据美国专利号5,338,671和5,587,287以及其他国家的相应专利批准用于体外研究的抗体。
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • L3289Lysis Solution for Blood化学品安全说明书
  • N9784Neutralization Solution for Blood化学品安全说明书
  • P8115Extract-N-Amp™ PCR ReadyMix™ for Blood化学品安全说明书

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P8115

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血液中和液, sufficient for 100 reactions, sufficient for 1000 reactions, for molecular biology

象形图

Corrosion
GHS05

警示用语：

Danger

危险声明

H290,H314

危险分类

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

储存分类代码

8A - Combustible corrosive hazardous materials

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

新产品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
SLCQ4678
SLCQ6412
SLCN6127
SLCC4699
SLBZ3480

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Roger G Rank et al.
Infection and immunity, 77(3), 1216-1221 (2009-01-14)
Over the last several years, four different phages of chlamydiae, in addition to a phage associated with Chlamydia psittaci isolated from an ornithosis infection in ducks over 25 years ago, have been described and characterized. While these phages and their
Min Lu et al.
Blood, 120(15), 3098-3105 (2012-08-09)
Interferon (IFN-α) is effective therapy for polycythemia vera (PV) patients, but it is frequently interrupted because of adverse events. To permit the long-term use of IFN, we propose combining low doses of IFN with Nutlin-3, an antagonist of MDM2, which
Bo G Winkel et al.
BMC medical genetics, 12, 22-22 (2011-02-11)
The use of dried blood spots (DBS) samples in genomic workup has been limited by the relative low amounts of genomic DNA (gDNA) they contain. It remains to be proven that whole genome amplified DNA (wgaDNA) from stored DBS samples
Maria C Walsh et al.
PloS one, 6(11), e27177-e27177 (2011-12-02)
We assessed the effect of short-term feeding of genetically modified (GM: Bt MON810) maize on immune responses and growth in weanling pigs and determined the fate of the transgenic DNA and protein in-vivo. Pigs were fed a diet containing 38.9%
Mads Vilhelm Hollegaard et al.
Molecular genetics and metabolism, 108(4), 225-231 (2013-02-21)
DNA methylation is the most common DNA modification and perhaps the best described epigenetic modification. It is believed to be important for genomic imprinting and gene regulation and has been associated with the development of diseases such as schizophrenia and
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商品

Extract-N-Amp™ Blood PCR Kit Protocol

The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

Extract-N-Amp Reagent: Effective PCR from FTA® Blood Cards

Extract-N-Amp simplifies DNA extraction and amplification, enabling PCR directly from FTA blood cards, reducing variability.

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Genomic DNA Purification and Assessment

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

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