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重组
expressed in E. coli
质量水平
方案
≥90% (SDS-PAGE)
表单
liquid
比活
≥35 units/mg protein
分子量
81 kDa
浓度
≥20 mg protein/mL (UV)
运输
wet ice
储存温度
2-8°C
生化/生理作用
Releases reducing sugars from birchwood xylan ( X0502), also catalyzes the hydrolysis of 4-methylumbelliferyl-β-D-cellobioside and 4-methylumbelliferyl-β-D-glucopyranoside. This enzyme does not possess endo-xylanase, arabinoxylanase or β-glucanase activities.
β-Xylosidase undergoes post-translation glycosylation which has been shown to be critical for its proper activity and stability. Deglycosylation altered the the optimum temperature and pH for activity and decreased its thermostability.
β-Xylosidase undergoes post-translation glycosylation which has been shown to be critical for its proper activity and stability. Deglycosylation altered the the optimum temperature and pH for activity and decreased its thermostability.
单位定义
One xylosidase unit will produce 1 μmole of o-nitrophenol per minute at 70 °C from
a 1mM solution of o-nitrophenyl-β-xyloside in 50 mM sodium acetate at pH 5.8.
a 1mM solution of o-nitrophenyl-β-xyloside in 50 mM sodium acetate at pH 5.8.
外形
Supplied as a solution in 50 mM Tris-HCl, pH 8.0, 100 mM NaCl, and 25% glycerol.
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Galina Mai-Gisondi et al.
Methods in molecular biology (Clifton, N.J.), 1588, 45-57 (2017-04-19)
Colorimetric detection of reaction products is typically preferred for initial surveys of acetyl xylan esterase (AcXE) activity. This chapter will describe common colorimetric methods, and variations thereof, for measuring AcXE activities on commercial, synthesized, and natural substrates. Whereas assays using
Alexandre Favarin Somera et al.
Journal of microbiology (Seoul, Korea), 47(3), 270-276 (2009-06-27)
Aspergillus versicolor grown on xylan or xylose produces two beta-xylosidases with differences in biochemical properties and degree of glycosylation. We investigated the alterations in the biochemical properties of these beta-xylosidases after deglycosylation with Endo-H or PNGase F. After deglycosylation, both
Sriwan Wongwisansri et al.
Bioresource technology, 132, 410-413 (2012-12-26)
A gene coding for thermotolerant β-xylosidase from Aspergillus sp. BCC125 was characterized. The recombinant enzyme was expressed in methylotrophic yeast Pichia pastoris KM71 and especially high yield of secreted enzyme was obtained. β-xylosidase possessed high enzyme efficiency (Kcat/Km=198.8mM(-1)s(-1)) toward pNP-β-D-xylopyranoside
Masahiro Kurakake et al.
Biotechnology letters, 33(12), 2453-2456 (2011-08-02)
Aspergillus awamori K4 β-xylosidase has broad acceptor specificity. It has been used to synthesize a sugar fatty acid ester via its transxylosylation activity. One xylosyl residue was initially transferred to hexamethylene glycol as a linker with a yield of 0.36
Lei Bao et al.
Applied biochemistry and biotechnology, 166(1), 72-86 (2011-10-25)
Two β-glucosidase/xylosidase genes, Rubg3A and Rubg3B, were cloned from yak rumen uncultured microorganisms by metagenome method and function-based screening. Recombinant RuBG3A and RuBG3B purified from Escherichia coli were characterized for enzymatic properties, and they exhibited activity against 4-nitrophenyl-β-D: -glucopyranoside and
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