生物来源
microbial
质量水平
形式
lyophilized powder
比活
≥7 units/mg solid
分子量
~160 kDa
技术
inhibition assay: suitable
颜色
brown
最佳pH
7.5-8.0
运输
wet ice
储存温度
−20°C
基因信息
Escherichia coli K12 ... xdhA(947116) , xdhB(947205) , xdhC(945148)
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一般描述
研究领域：细胞周期
黄嘌呤氧化酶(XO)，一种多功能的含钼黄素蛋白，是黄嘌呤氧化还原酶(XORs)的形式之一。每个黄嘌呤氧化酶由2个黄素分子(FAD)、2个钼原子和8个铁原子构成。
黄嘌呤氧化酶(XO)，一种多功能的含钼黄素蛋白，是黄嘌呤氧化还原酶(XORs)的形式之一。每个黄嘌呤氧化酶由2个黄素分子(FAD)、2个钼原子和8个铁原子构成。
应用
黄嘌呤氧化酶（微生物）可用于：
- 测定体外黄嘌呤氧化酶活性
- 在超氧化物歧化酶检测中生成超氧阴离子
- 作为比色纸装置的反应混合液组分，酶法检测传统药物中的别嘌呤醇
生化/生理作用
黄嘌呤氧化酶在嘌呤代谢中催化次黄嘌呤羟基化为黄嘌呤，黄嘌呤羟基化为尿酸和氧自由基。这种酶也参与咖啡因代谢。黄嘌呤氧化酶在药物等异源物质的代谢中也起重要作用，例如硫嘌呤类药物，涉及6-巯基嘌呤、别嘌呤醇和尿酸。黄嘌呤氧化酶活性过高会引起高尿酸血症（血中尿酸过多），进一步导致痛风。
还含有蛋白水解活性。
物理属性
抑制剂：还原剂，Hg++，Ag+，MIA
最佳pH: 7.5 - 8.0
最佳温度：65oC
pH稳定性：pH 6.5 - 9.0 (25oC, 15hr)
温度稳定性：低于55oC (pH 8.0, 30min)
最佳pH: 7.5 - 8.0
最佳温度：65oC
pH稳定性：pH 6.5 - 9.0 (25oC, 15hr)
温度稳定性：低于55oC (pH 8.0, 30min)
单位定义
在25 °C pH 7.5条件下，一个单位可在每分钟内将1.0 μ摩尔的黄嘌吟转化为尿酸。在次黄嘌呤作为底物时可获得约50%的活性。
外形
含有BSA和谷氨酸钠作为稳定剂的冻干粉末
储存及稳定性
Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons
persons
抑制剂
警示用语：
Danger
危险声明
危险分类
Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
含少量动物源组分生物产品
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实验方案
The appearance of uric acid is measured at 293nm by spectrophotometry.
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