生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen 85 kDa
种属反应性
human
浓度
~1 mg/mL
技术
indirect immunofluorescence: 5-10 μg/mL using MCF7 cells fixed with paraformaldehyde-Triton
western blot: 0.5-1 μg/mL using A431 cell lysates
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... XRCC1(7515)
mouse ... Xrcc1(22594)
rat ... Xrcc1(84495)
一般描述
X-ray repair cross-complementing group 1 (XRCC1) is a testis enriched protein. It is considered as a DNA repair gene.
免疫原
synthetic peptide corresponding to amino acids 615-633 of human XRCC1, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is identical in mouse and rat.
应用
Anti-XRCC1 antibody produced in rabbit has been used in:
- immunoblotting
- immunofluorescence
- indirect immunofluorescence and telomere FISH (TEL-FISH)
- protein detection
- western analysis
生化/生理作用
X-ray repair cross-complementing group 1 (XRCC1) is a molecular scaffold protein that coordinates the assembly of repair complexes at damaged sites. XRCC has been shown to physically interact with several enzymes known to be involved in the repair of SSBs, including DNA ligase IIIa, DNA polymerase b, APE1, polynucleotide kinase (PNK), poly (ADP-ribose), polymerases 1 and 2 (PARP-1 and 2) and others. It participates in the DNA base excision repair (BER) pathway.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
Versatile recombinant SUMOylation system for the production of SUMO-modified protein
Weber AR, et al.
PLoS ONE, 9(7), e102157-e102157 (2014)
XRCC1 polymorphism and overall survival in ovarian cancer patients treated with platinum-based chemotherapy: A systematic review and MOOSE-compliant meta-analysis
Zhang Z, et al.
Medicine, 97(45) (2018)
X-ray repair cross complementing protein 1 in base excision repair
Hanssen-Bauer A, et al.
International Journal of Molecular Sciences, 13(12), 17210-17229 (2012)
XRCC1 deficiency correlates with increased DNA damage and male infertility
Singh V, et al.
Mutation Research. Genetic Toxicology and Environmental Mutagenesis, 839 (2019)
Simon D Schwarz et al.
Nature communications, 15(1), 184-184 (2024-01-04)
The intracellular ATP-ribosyltransferases PARP1 and PARP2, contribute to DNA base excision repair (BER) and DNA demethylation and have been implicated in epigenetic programming in early mammalian development. Recently, proteomic analyses identified BER proteins to be covalently poly-ADP-ribosylated by PARPs. The
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