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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
3H8, monoclonal
形式
buffered aqueous solution
种属反应性
human
技术
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: suitable
western blot: 1-5 μg/mL
同位素/亚型
IgG1κ
GenBank登记号
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... PTBP1(5725)
一般描述
该基因属于普遍表达的异质核糖核蛋白（hnRNPs）的亚家族。hnRNP是RNA结合蛋白，其与异质核RNA（hnRNA）形成复合物。这些蛋白质与细胞核中的前mRNA相关，并且似乎影响前mRNA的加工以及mRNA代谢和转运的其他方面。尽管所有hnRNP存在于细胞核中，但有些似乎在细胞核和细胞质之间穿梭。hnRNP蛋白具有独特的核酸结合特性。该基因编码的蛋白质具有结合RNA的准RNA识别基序（RRM）域的四个重复序列。该蛋白质与需要前mRNA剪接的内含子聚嘧啶束结合，并通过蛋白质降解泛素-蛋白酶体途径起作用。它也可能促进U2 snRNP与前mRNA的结合。该蛋白位于核质中，也可以在核仁周围结构中检测到。已经描述了编码不同亚型的选择性剪接的转录变体。（由RefSeq提供）
免疫原
PTBP1（NP_002810, 45 a.a.~ 144 a.a）带有GST标签的部分重组蛋白。仅GST标签的MW为26 KDa。
序列
KKFKGDSRSAGVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIEMNTEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDSSPNQ
序列
KKFKGDSRSAGVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIEMNTEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDSSPNQ
外形
溶于pH7.4的磷酸盐缓冲盐水中的溶液
法律信息
GenBank is a registered trademark of United States Department of Health and Human Services
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
Alfredo Castello et al.
Nature protocols, 8(3), 491-500 (2013-02-16)
Owing to their preeminent biological functions, the repertoire of expressed RNA-binding proteins (RBPs) and their activity states are highly informative about cellular systems. We have developed a novel and unbiased technique, called interactome capture, for identifying the active RBPs of
Endothelial deletion of PTBP1 disrupts ventricular chamber development.
Liu, et al.
Nature Communications, 14, 1796-1796 (2023)
Global analysis of RNA-binding protein dynamics by comparative and enhanced RNA interactome capture.
Joel I Perez-Perri et al.
Nature protocols, 16(1), 27-60 (2020-11-20)
Interactions between RNA-binding proteins (RBPs) and RNAs are critical to cell biology. However, methods to comprehensively and quantitatively assess these interactions within cells were lacking. RNA interactome capture (RIC) uses in vivo UV crosslinking, oligo(dT) capture, and proteomics to identify
Raul Guantes et al.
Genome research, 25(5), 633-644 (2015-03-25)
Noise in gene expression is a main determinant of phenotypic variability. Increasing experimental evidence suggests that genome-wide cellular constraints largely contribute to the heterogeneity observed in gene products. It is still unclear, however, which global factors affect gene expression noise
Samantha M Grist et al.
Nature communications, 11(1), 6237-6237 (2020-12-06)
Immunoassays and mass spectrometry are powerful single-cell protein analysis tools; however, interfacing and throughput bottlenecks remain. Here, we introduce three-dimensional single-cell immunoblots to detect both cytosolic and nuclear proteins. The 3D microfluidic device is a photoactive polyacrylamide gel with a
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