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安全信息

W1878

SAFC

Williams′ 培养基E

With sodium bicarbonate, without ʟ-glutamine and phenol red, sterile liquid, suitable for cell culture

制药生产

别名:

Williams’ E medium

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.75

product name

Williams′ 培养基E, With sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

质量水平

400

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

sodium pyruvate: 0.025 g/L
phenol red: no
glucose: 2.0 g/L (Dextro)
NaHCO3: 2.2 g/L
L-glutamine: no

运输

ambient

储存温度

2-8°C

相关类别

一般描述

Williams培养基E由Williams和Gunn在Williams培养基D的基础上改良而成。培养基专用于支持成人肝脏上皮细胞的长期细胞培养。它是改良型最低必需培养基(MEM)，改变了葡萄糖和氨基酸的含量。

应用

Williams培养基E已用于培养人肝细胞癌(HCC)细胞和肝细胞。

重悬

添加 0.292g/L L-谷氨酰胺。

其他说明

酚红会干扰一些细胞在低密度或克隆密度下的生长。在使用干细胞或低密度细胞时，请使用这款William's E。

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WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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Nicole A Kratochwil et al.
The AAPS journal, 19(2), 534-550 (2017-01-05)
Early prediction of human clearance is often challenging, in particular for the growing number of low-clearance compounds. Long-term in vitro models have been developed which enable sophisticated hepatic drug disposition studies and improved clearance predictions. Here, the cell line HepG2
Robert D Mitchell et al.
Journal of biochemical and molecular toxicology, 30(8), 375-395 (2016-04-20)
New paradigms for human health risk assessment of environmental chemicals emphasize the use of molecular methods and human-derived cell lines. In this study, we examined the effects of the insect repellent DEET (N,N-diethyl-m-toluamide) and the phenylpyrazole insecticide fipronil (fluocyanobenpyrazole) on
Dariusz Ratman et al.
Nucleic acids research, 44(22), 10539-10553 (2016-09-01)
Adaptation to fasting involves both Glucocorticoid Receptor (GRα) and Peroxisome Proliferator-Activated Receptor α (PPARα) activation. Given both receptors can physically interact we investigated the possibility of a genome-wide cross-talk between activated GR and PPARα, using ChIP- and RNA-seq in primary
Ezgi Eyluel Bankoglu et al.
PloS one, 11(11), e0166956-e0166956 (2016-11-29)
Type 2 diabetes (T2DM) and obesity are frequently associated with non-alcoholic fatty liver disease (NAFLD) and with an elevated cancer incidence. The molecular mechanisms of carcinogenesis in this context are only partially understood. High blood insulin levels are typical in
Lor Huai Chong et al.
Lab on a chip, 18(21), 3239-3250 (2018-09-27)
Drug-induced skin sensitization is prevalent worldwide and can trigger life-threatening health conditions, such as Stevens Johnson Syndrome. However, existing in vitro skin models cannot adequately predict the skin sensitization effects of drugs administered into the systemic circulation because dermal inflammation
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