product name
V5 肽, ≥97% (HPLC), lyophilized powder
质量水平
检测方案
≥97% (HPLC)
形式
lyophilized powder
分子量
calculated mol wt 1524.78 Da
储存温度
−20°C
Amino Acid Sequence
Cys-Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr
一般描述
V5 标签肽是一种合成肽，其氨基酸序列对应于非结构蛋白 V 的氨基酸 95-108 和副粘病毒 SV5 的 RNA 聚合酶 a 亚基（P 蛋白），具有 N-末端半胱氨酸。
应用
该肽在免疫测定中用于从抗 V5 抗体置换 V5 标记的融合蛋白。成功抑制抗体与 V5 肽的结合表明结合具有特异性。
其他说明
氨基酸序列相当于非结构蛋白 V 的氨基酸 95-109 和 RNA 聚合酶 α副粘病毒 SV5 的亚基（P 蛋白），具有 N-末端半胱氨酸。
从0.1% TFA水溶液中冻干
氨基酸序列为 N-Cys-Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-C
制备说明
为制备贮备液，用水溶解至终浓度 5 mg/mL。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
Eden A Dejene et al.
The Journal of biological chemistry, 295(30), 10255-10270 (2020-05-28)
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Yan-ling Zhang et al.
Archives of biochemistry and biophysics, 483(1), 45-54 (2009-01-06)
Tissue specific amplification of glucocorticoid action through NADPH-dependent reduction of inactive glucocorticoid precursors by 11beta-hydroxysteroid dehydrogenase (11beta-HSD1) contributes to the development of visceral obesity, insulin resistance and Type 2 Diabetes. Hexose-6-phosphate dehydrogenase (H6PDH) is believed to supply NADPH for the
Rui Chen et al.
PloS one, 12(12), e0190241-e0190241 (2017-12-28)
Survival of cancer cells in the harsh tumor microenvironment, characterized by oxygen and glucose deprivation, requires rapid initiation of cytoprotective measures. Metabolites whose levels change during stress are ideal signaling cues, particularly if used in post-translational modifications of stress-responsive signal
Paul Yenerall et al.
Cell chemical biology, 27(1), 105-121 (2019-12-31)
RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer. Here we investigated the molecular and phenotypic role of RUVBL1/2 ATPase activity in non-small cell lung cancer (NSCLC). We find that RUVBL1/2
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