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Merck
CN

V6630

Anti-Vimentin Antibody

mouse monoclonal, V9

别名:

单克隆抗波形蛋白抗体

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UNSPSC Code:
12352203
NACRES:
NA.41

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产品名称

波形蛋白单克隆抗体 小鼠抗, clone V9, ascites fluid

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

V9, monoclonal

mol wt

antigen ~58 kDa

contains

15 mM sodium azide

species reactivity

pig, canine, feline, hamster, rabbit, gerbil, monkey, bovine, chicken, human, horse, rat

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tissue
western blot: 1:200 using human fibroblasts HS-68

isotype

IgG1

UniProt accession no.

P08670

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... VIM(7431)
rat ... Vim(81818)

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Application

单克隆抗波形蛋白抗体已用于免疫印迹和免疫细胞化学。
小鼠产生的单克隆抗波形蛋白抗体已用于：
  • 免疫荧光分析
  • 免疫印迹
  • 抗波形蛋白抗体在胚胎操作前注入成熟卵母细胞，结合内源性波形蛋白 (VIM)
  • 免疫细胞化学中
小鼠来源抗波形蛋白单克隆抗体可用作一抗以通过免疫组化 (IHC) 定位波形蛋白，还可用于波形蛋白的免疫沉淀。

Biochem/physiol Actions

在免疫组织化学染色中，抗体将波形蛋白定位于成纤维细胞、内皮细胞、淋巴组织和黑色素细胞中。它也在肉瘤、淋巴瘤和黑色素瘤中染色波形蛋白。
在前列腺癌、乳腺癌、子宫内膜癌、中枢神经系统（CNS）肿瘤、恶性黑色素瘤和胃肠道肿瘤（包括胰腺癌、结直肠癌和肝癌）细胞中，均观察到波形蛋白表达上调。因此，波形蛋白可以作为开发癌症疗法的靶标。在血管内皮生长因子受体 1 (VEGFR-1) 诱导的蛋白激酶 G (PKG) 1 信号传导通路激活（刺激心肌细胞肥大消退）中，波形蛋白发挥着至关重要的作用。波形蛋白丝参与多种生理过程，包括迁移、细胞形状维持和间充质细胞的机械应力耐受。波形蛋白与 LARP6（La 核糖核蛋白域家族成员 6）相互作用并稳定 I 型胶原 mRNA，可能在组织纤维化的发展中发挥重要作用。波形蛋白在维持晶状体完整性中起着至关重要的作用，因此，波形蛋白基因突变会导致显性白内障和粉状白内障。
波形蛋白是未成熟神经胶质细胞的主要细胞骨架成分。它是一种中间丝蛋白，可作为多种半胱天冬酶的底物。半胱天冬酶裂解该蛋白时，会分解中间丝并促进细胞凋亡。
波形蛋白有助于保持间充质细胞的细胞结构和组织稳定性。它参与肿瘤发生、EMT（上皮间质转化）和癌症的转移扩散，控制人乳头瘤病毒16型假病毒的感染内化。

Disclaimer

除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或人类或动物应用。

General description

波形蛋白由人类染色体10p13上的单拷贝基因编码。53kDa的波形蛋白属于III类中间纤维(IF)蛋白质家族，由正常间质细胞表达。含466个氨基酸的波形蛋白特征在于高度保守的a-螺旋“棒状” 结构域，该结构域两侧为非α-螺旋氨基- 末端 “头” 和羧基末端 “尾” 结构域。

Immunogen

猪眼晶状体波形蛋白。

Other Notes

本品为采用细胞培养杂交瘤生产的纯化产品
V6389抗波形蛋白抗体，小鼠单克隆
克隆V9，从杂交瘤细胞培养基中纯化

Preparation Note

需要连续使用时，可在 2-8°C 下储存一个月。对于更长期的储存，溶液可以冷冻成工作等分试样。不建议重复冷冻和解冻。不建议储存在“无霜”冰箱中。如果长期储存时出现轻微混浊，使用前通过离心澄清。

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存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000492460
0000492460
0000446959
0000446959
0000421314

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the epitope sequence for product V6630, Monoclonal Anti-Vimentin antibody produced in mouse?

    Product V6630, Monoclonal Anti-Vimentin antibody, is a licensed antibody. The development and specification of this clone are specified in: Osborn, M., et al., Europ. J. Cell Biol., 34 137-143 (1984). In this paper the immunogen is porcine vimentin.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

D Dahl et al.
European journal of cell biology, 24(2), 191-196 (1981-06-01)
Comparison of cytoskeletal preparations obtained from newborn and adult rat brain showed similar patterns on SDS-PAGE. However, coelectrophoresis of the newborn and adult preparations revealed distinct differences in the mobility of 2 major bands in the molecular weight range of
Bupivacaine-induced regeneration of rat soleus muscle: ultrastructural and immunohistochemical aspects.
Politi PK, et al.
Ultrastructural Pathology, 30(6), 461-469 (2006)
Behzad Gerami-Naini et al.
Endocrinology, 145(4), 1517-1524 (2003-12-20)
Trophoblast differentiation and early placental development are essential for the establishment of pregnancy, yet these critical events are not readily investigated in human pregnancy. We used embryoid bodies (EBs) prepared from human embryonic stem (hES) cells as an in vitro
Eirik Grønevik et al.
The journal of gene medicine, 5(10), 909-917 (2003-10-09)
Genes encoding non-self proteins may be injected into skeletal muscles in vivo to obtain induction of cellular and humoral immune responses against the encoded antigens (DNA vaccination). Bone marrow derived professional antigen-presenting cells (APCs) play a key role in the
Kei Miyamoto et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(17), 7040-7045 (2011-04-13)
Nuclear reprogramming of differentiated cells can be induced by oocyte factors. Despite numerous attempts, these factors and mechanisms responsible for successful reprogramming remain elusive. Here, we identify one such factor, necessary for the development of nuclear transfer embryos, using porcine
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包含β-肌动蛋白、α-微管蛋白和波形蛋白用于细胞骨架结果蛋白活细胞分析的高滴度慢病毒颗粒。

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