产品名称
抗-VSV糖蛋白单克隆抗体 小鼠抗, clone P5D4, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
P5D4, monoclonal
contains
15 mM sodium azide
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1:100,000 using whole cell extracts expressing VSV-G tagged fusion protein
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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Preparation Note
如需连续使用，可在2-8 °C保存至多一月。若需延长储存时间，可将溶液分装并冷冻。 不建议反复冻融。不建议在无霜冰箱中储存。如果长期储存后发生轻微浑浊，请在使用前通过离心澄清溶液。稀释的工作溶液在12小时后应当被丢弃。
Application
成功使用该抗体的应用以及相关同行评审论文如下所示。
流式细胞术/细胞分选（1 篇）
流式细胞术/细胞分选（1 篇）
Biochem/physiol Actions
该抗体可识别包含VSV糖蛋白的五个羧基末端氨基酸的表位。在受感染的细胞中，该抗体将未成熟型VSV-G定位在粗糙内质网（RER）和高尔基复合体潴泡中，将成熟型VSV-G定位在细胞表面和出芽病毒中。该抗体不会染色缺少膜和细胞质结构域的分泌型VSV-G。该抗体可用于微注射抗体、免疫印迹、免疫沉淀、免疫细胞化学和免疫电镜法研究新合成的VSV-G在向质膜和细胞表面转移过程中细胞质结构域的作用。该抗体可用于对以VSV-G羧基末端标记的外源性导入结构进行检测、免疫沉淀和免疫细胞化学染色。该标记不会干扰所研究蛋白质的功能，可被P5D4抗体特异性识别，而不与任何内源性蛋白质发生交叉反应。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Immunogen
KLH标记的含有水泡性口炎病毒糖蛋白（VSV-G）的15个羧基末端氨基酸（497-511）的合成肽。
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
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