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安全信息

V4888

Sigma-Aldrich

抗 VSV-G 兔抗

affinity isolated antibody, buffered aqueous solution

别名:

抗 水泡性口炎病毒糖蛋白

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100 μL
¥5,551.31

¥5,551.31

国内现货，预计发货时间2025年4月16日详情

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100 μL
¥5,551.31

About This Item

MDL编号:
MFCD00164761
UNSPSC代码:
12352203
NACRES:
NA.56

¥5,551.31

国内现货，预计发货时间2025年4月16日详情

生物来源

rabbit

偶联物

unconjugated

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

表单

buffered aqueous solution

种属反应性

mammals

浓度

~1.0 mg/mL

技术

immunoprecipitation (IP): 0.5 μg using VSV-G tagged fusion proteins from transfected mammalian cell lysates (tagged fusion proteins from transfected mammalian cell lysates)
indirect immunofluorescence: 1.0 μg/mL using VSV-G tagged fusion proteins in methanol/acetone fixed transiently transfected cells
western blot: 0.1-0.2 μg/mL using VSV-G tagged fusion proteins from transfected mammalian cell lysates

运输

dry ice

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Anti-NCAPH antibody produced in rabbit Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

HPA003008

Anti-NCAPH antibody produced in rabbit

Anti-NCAPH antibody produced in rabbit affinity isolated antibody

SAB4500982

Anti-NCAPH antibody produced in rabbit

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

product line

Prestige Antibodies® Powered by Atlas Antibodies

product line

-

product line

Prestige Antibodies® Powered by Atlas Antibodies

product line

-

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

一般描述

抗 VSV-G 兔抗体与氨基端和羧基端 VSV-G 标记的融合蛋白反应。
水泡性口炎病毒（VSV）属于弹状病毒科（Rhabdoviridae）的水泡病毒属（Vesiculovirus）。[1]水泡性口炎病毒G（VSV G）蛋白被认为是早期鉴定出的III型病毒融合蛋白。[2]

免疫原

与水疱性口炎病毒糖蛋白 (VSV-G) 的氨基酸 497-511 相应的 N-Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys-C）相对应的合成肽，通过在羧基端添加半胱氨酸残基与马来酰亚胺结合活化 KLH。该抗体可识别 C 端和 N 端的 VSV-G 标签。

应用

兔生产的抗VSV-G 抗体已被用于免疫印迹[3][4]和免疫沉淀。[5][6]
成功使用该抗体的应用以及相关同行评审论文如下所示。
染色质免疫沉淀（1 篇）
蛋白免疫印迹（1 篇）
免疫沉淀后的蛋白免疫印迹（1 篇）
抗体适用于免疫印迹、免疫沉淀和间接免疫荧光。
免疫组化。

生化/生理作用

在低pH刺激下，水泡性口炎病毒G（VSV G）能够刺激细胞融合。VSV G蛋白的跨膜区对于病毒从半融合状态向完全融合状态的转变至关重要。[2]

外形

溶于含有1%牛血清白蛋白和15mM叠氮化钠（作为防腐剂）的0.01M磷酸盐缓冲盐水（pH7.4）中。

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储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品
  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    0000414612
    0000414612
    0000380705
    0000380705
    0000341559

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    Vesicular stomatitis virus glycoprotein is a determinant of pathogenesis in swine, a natural host
    Martinez I, et al.
    Journal of virology, 77(14), 8039-8047 (2003)
    Vesicular stomatitis virus G protein transmembrane region is crucial for the hemi-fusion to full fusion transition
    Ci Y, et al.
    Scientific Reports, 8(1), 10669-10669 (2018)
    Periodic fever with aphthous stomatitis, pharyngitis, and cervical adenitis syndrome is associated with a CARD8 variant unable to bind the NLRP3 inflammasome
    Cheung MS, et al.
    Journal of immunology (Baltimore, Md. : 1950), 198(5), 2063-2069 (2017)
    Sudha Chugani et al.
    Proceedings of the National Academy of Sciences of the United States of America, 107(23), 10673-10678 (2010-05-26)
    Pseudomonas aeruginosa quorum control of gene expression involves three LuxR-type signal receptors LasR, RhlR, and QscR that respond to the LasI- and RhlI-generated acyl-homoserine lactone (acyl-HSL) signals 3OC12-HSL and C4-HSL. We found that a LasR-RhlR-QscR triple mutant responds to acyl-HSLs
    Adenomatous polyposis coli (APC) regulates multiple signaling pathways by enhancing glycogen synthase kinase-3 (GSK-3) activity
    Valvezan AJ, et al.
    The Journal of Biological Chemistry, 287(6), 3823-3832 (2012)
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