V2260
单克隆抗- V5−过氧物酶 小鼠抗
clone V5-10, purified immunoglobulin, lyophilized powder
别名:
单克隆抗-V5
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关于此项目
Conjugate:
peroxidase conjugate
Clone:
V5-10, monoclonal
Application:
WB
Citations:
14
storage temp.
2-8°C
Quality Level
biological source
mouse
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
V5-10, monoclonal
form
lyophilized powder
packaging
vial of 0.5 mL
concentration
5-11 mg/mL
technique(s)
western blot: 1:4,000-1:8,000
General description
V5 标签是一个 14 个氨基酸的长肽序列（GKPIPNPLGLGST），来源于猴病毒 5（SV5）的 P 和 V 蛋白上的表位，属于副粘病毒家族。单克隆抗 V5 过氧化物酶偶联物与在转染的哺乳动物细胞中表达或通过体外翻译产生的带有 V5 标签的重组融合蛋白反应。
Application
适用于免疫印迹。
Biochem/physiol Actions
识别 V5 标签（GKPIPNPLLGLDST）融合蛋白
Physical form
从含有1%BSA和0.05%MIT的0.01 M磷酸盐缓冲盐溶液（pH 7.4）中冻干
Preparation Note
制备两步戊二醛方法通过Avrameas, S., et al., Scand.J. Immunol., 8, Suppl. 7, 7 (1978)描述。
用 0.5 mL 无菌蒸馏水复溶。
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Panayotis C Theodoropoulos et al.
Nature chemical biology, 12(4), 218-225 (2016-02-02)
A hallmark of targeted cancer therapies is selective toxicity among cancer cell lines. We evaluated results from a viability screen of over 200,000 small molecules to identify two chemical series, oxalamides and benzothiazoles, that were selectively toxic at low nanomolar
Jordan N Ranum et al.
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Productive infections by RNA viruses require faithful replication of the entire genome. Yet many RNA viruses also produce deletion-containing viral genomes (DelVGs), aberrant replication products with large internal deletions. DelVGs interfere with the replication of wild-type virus and their presence
Shuang Li et al.
iScience, 27(5), 109646-109646 (2024-04-19)
Most advanced colorectal cancer (CRC) patients cannot benefit from targeted therapy due to lack of actionable targets. By mining data from the DepMap, we identified FAM126B as a specific vulnerability in CRC cell lines exhibiting low FAM126A expression. Employing a
Vy Tran et al.
Nature microbiology, 5(12), 1490-1503 (2020-08-26)
Cells infected by influenza virus mount a large-scale antiviral response and most cells ultimately initiate cell-death pathways in an attempt to suppress viral replication. We performed a CRISPR-Cas9-knockout selection designed to identify host factors required for replication after viral entry.
Steven F Baker et al.
Cell reports, 24(10), 2581-2588 (2018-09-06)
Adaptation of viruses to their hosts can result in specialization and a restricted host range. Species-specific polymorphisms in the influenza virus polymerase restrict its host range during transmission from birds to mammals. ANP32A was recently identified as a cellular co-factor
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