生物来源
bakers yeast
质量水平
类型
Type X
形式
lyophilized powder
比活
≥40 units/mg protein
组成
Protein, 30-60% modified Warburg-Christian
异质活性
UDP-glucose dehydrogenase and galactose-1-phosphate uridyltransferase ≤0.1%
inorganic pyrophosphatase ≤0.5%
储存温度
−20°C
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一般描述
尿苷-5′-二磷酸葡萄糖焦磷酸化酶（UDP-glc-PPase）普遍存在于植物、酵母、细菌和哺乳动物中。这种酶是由八个相同亚基组成的八聚体。
应用
来自面包酵母的尿苷-5′-二磷酸葡萄糖焦磷酸化酶′已用于：
- 合成尿苷-5′-二磷酸葡萄糖(UDP-Glc)-13C9
- 量化水稻中蔗糖合酶（SUS）的活性
- 研究己糖激酶和糖原合成酶控制青蛙卵母细胞通量的作用
生化/生理作用
尿苷-5′-二磷酸葡萄糖焦磷酸化酶（UDP-glc-PPase）参与催化UDP-葡萄糖的合成。这种酶的活性需要二价阳离子，例如Mg2+、Ca2+、Mn2+、Ni2+。
单位定义
一个单位将在25℃和pH 7.6下，每分钟由尿苷-5′-二磷酸葡萄糖和无机焦磷酸盐形成1.0 μmole的1-磷酸葡萄糖。
外形
冻干粉，不含硫酸盐，含柠檬酸缓冲盐
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
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Kieran Smallbone et al.
Methods in enzymology, 500, 355-370 (2011-09-29)
In this chapter, we describe the steps needed to create a kinetic model of a metabolic pathway based on kinetic data from experimental measurements and literature review. Our methodology is presented by utilizing the example of trehalose metabolism in yeast.
Himangi R Jayakar et al.
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A number of studies have revealed that Francisella tularensis (FT) suppresses innate immune responses such as chemokine/cytokine production and neutrophil recruitment in the lungs following pulmonary infection via an unidentified mechanism. The ability of FT to evade early innate immune
Zhe Ma et al.
Molecular biology reports, 38(4), 2751-2760 (2010-11-26)
UDP-Glucose Pyrophosphorylase (EC 2.7.7.9, UGPase) plays an important role in Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) cell envelope Hyaluronic acid (HA) biosynthesis and it is also recognized as a virulence determinant in several bacterial species. HA is valuable biopolymer used
D J Sutor et al.
Clinica chimica acta; international journal of clinical chemistry, 86(3), 329-332 (1978-06-15)
The concentration of pyrophosphate in urine has been determined using the enzyme uridine-5'-diphosphoglucose pyrophosphorylase. The technique is relatively quick and easy to perform. Reproducibility of results was good, and results from recovery experiments were excellent. The mean concentration of pyrophosphate
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