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形式
lyophilized powder
质量水平
比活
15-30 units/mg protein (biuret)
分子量
tetramer ~32 kDa
储存温度
−20°C
InChI
1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3
InChI key
VPXDHZMLJOJKOX-UHFFFAOYSA-N
一般描述
Research area: Cell Signalling. Uricase is made of four identical subunits and is a homotetrameric enzyme. It is found as a functional uricase in several organisms including, bacteria, archaea, and eukaryotes. Nonetheless, primates and humans lack a functional uricase enzyme.(2)
应用
用 球状节杆菌 产生的尿酸酶涂覆电极制成生物传感器，可检测尿样中的尿酸。它也可用于分析 Zn、Cu、Mg 和 Mn 离子对尿酸酶的影响。
生化/生理作用
球形节杆菌是家禽粪便和垃圾中尿酸酶或尿酸氧化酶的主要产生菌。尿酸酶可水解尿酸，引起禽粪氨挥发。在大多数哺乳动物中，尿酸酶将尿酸转化为尿囊素（一种可溶性排泄产物）。人类缺乏尿酸酶是由于在原始人类进化过程中丢失了该酶。由于这增加了尿酸循环水平，人类罹患痛风、肾结石、高血压和糖尿病的风险也随之加大。
Zn 和 Cu 显示能抑制尿酸酶活性。
分子量： 4×~32 kDa （四聚体）
等电点：4.64
K M 值（以尿酸为底物）：~0.01 mM
活性的最适 pH：8.5-9.5
稳定性最佳 pH 值：6.0-9.5
等电点：4.64
K M 值（以尿酸为底物）：~0.01 mM
活性的最适 pH：8.5-9.5
稳定性最佳 pH 值：6.0-9.5
单位定义
在25℃、pH 9.0条件下，一个单位每分钟可将 1.0μ摩尔尿酸转化为尿囊素。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
Development of a sandwich format, amperometric screen-printed uric acid biosensor for urine analysis.
Kanyong P, et al.
Analytical biochemistry, 428(1), 39-43 (2012)
Review of Uricase and Other Key Metabolites Produced by Microorganisms through Submerged Fermentation INTRODUCTION
Sandeep VC, et al.
Polymer Journal, 22, 1500-1517 (2023)
Uric acid: a danger signal from the RNA world that may have a role in the epidemic of obesity, metabolic syndrome, and cardiorenal disease: evolutionary considerations.
Seminars in Nephrology, 31(5), 394-399 (2011)
A Therapeutic Uricase with Reduced Immunogenicity Risk and Improved Development Properties
Andrew C N, et al.
PLoS ONE, 11(12) (2016)
Kavita R Hegde et al.
Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift fur Augenheilkunde, 222(3), 194-198 (2008-05-24)
Oxidative stress is considered to be a significant risk factor in the pathogenesis of many eye diseases associated with aging. The purpose of this study was to investigate the susceptibility of retinal tissue to reactive oxygen species (ROS)-induced damage, and
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