重组
expressed in Sf9 cells
质量水平
形式
buffered aqueous glycerol solution
分子量
96 kDa
浓度
≥0.20 mg/mL protein (Bradford)
≥51% (SDS-PAGE)
NCBI登记号
运输
dry ice
储存温度
−70°C
一般描述
Human USP5 (Ubiquitin-Specific Protease 5) or Isopeptidase T (isoT), amino acids 2-858 (end) with an N-terminal FLAG-tag, expressed in a baculovirus-infected Sf9 cell expression system.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
生化/生理作用
The tumor suppressor gene P53 was shown to be a target of ubiquitin-specific protease 5.
外形
Solution in 40 mM Tris-HCl, 110 mM NaCl, 2.2 mM KCl, 80 ng/μl Flag peptide, 3 mM DTT, and 20% Glycerol at pH 8.0.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
The Journal of biological chemistry, 283(28), 19581-19592 (2008-05-17)
The conjugation of polyubiquitin to target proteins acts as a signal that regulates target stability, localization, and function. Several ubiquitin binding domains have been described, and while much is known about ubiquitin binding to the isolated domains, little is known
The Journal of biological chemistry, 284(8), 5030-5041 (2008-12-23)
Both p53 and its repressor Mdm2 are subject to ubiquitination and proteasomal degradation. We show that knockdown of the deubiquitinating enzyme USP5 (isopeptidase T) results in an increase in the level and transcriptional activity of p53. Suppression of USP5 stabilizes
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