生物来源
human
质量水平
重组
expressed in E. coli (N-terminal histidine tagged)
检测方案
≥95% (GE)
形式
lyophilized powder
分子量
10.7 kDa
技术
ligand binding assay: suitable
溶解性
Tris-HCl, pH 7.5: 1.0—1.10 mg/mL, clear to slightly hazy, colorless
适用性
suitable for molecular biology
储存温度
−20°C
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一般描述
泛素是球形蛋白，由 76 个氨基酸组成，表面具有赖氨酸残基。分子量为 8565 Da。
Research Area: CANCER
Ubiquitin is a highly conserved protein composed of 76 amino acids, and it is expressed universally in all eukaryotes, ranging from yeast to humans.
Ubiquitin is a highly conserved protein composed of 76 amino acids, and it is expressed universally in all eukaryotes, ranging from yeast to humans.
应用
人泛素可作为研究水稻 E3-Ubiquitin 连接酶的分离、鉴定及 OsHOS1 基因在冷应激反应调控中的作用的供试化合物。
泛素（人）可用于：
- 作为底物，用于 体外 泛素化测定
- 作为底物，用于ADP-核糖基化和泛素化测定
- 向新鲜血液补充 64 ng内源性泛素，用于实验分组，研究其对癌症预后的临床潜在影响
生化/生理作用
泛素对真核细胞发挥正常功能起关键作用。参与免疫系统发育和免疫系统功能正常运行。此外，此免疫亲和蛋白还参与调节免疫稳态。泛素肽片段（U50-59）能够阻断细胞免疫和体液免疫。
泛素是存在于真核生物组织中的一种小分子调节蛋白。外源性泛素可以刺激许多细胞系凋亡。人乳头状瘤病毒-16 的 E7 蛋白通过泛素-蛋白酶体途径刺激视网膜母细胞瘤蛋白降解。
Ubiquitin-mediated proteolysis plays an important role in several basic cellular processes including regulation of cell cycle and division, differentiation and development, modulation of cell-surface receptors, the secretory pathway (protein transport), morphogenesis of neuronal networks, transcriptional regulation and signal transduction, transcriptional silencing, DNA repair, long-term memory, and circadian rhythms.
制备说明
泛素人可溶于 0.02 MTris-HCl，浓度为 1.00-1.10 mg/mL，得到澄清至略微浑浊的无色溶液。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
Ubiquitin modification by the E3 ligase/ADP-ribosyltransferase Dtx3L/Parp9
Yang CS, et al.
Molecular Cell, 66(4), 503-516 (2017)
Tiago Lourenço et al.
Plant molecular biology, 83(4-5), 351-363 (2013-06-20)
Plants can cope with adverse environmental conditions through the activation of stress response signalling pathways, in which the proteasome seems to play an important role. However, the mechanisms underlying the proteasome-mediated stress response in rice are still not fully understood.
Regulation of NF-kappaB by ubiquitination
Chen J and Chen ZJ
Current Opinion in Immunology, 4-12 (2013)
New crystal form of human ubiquitin in the presence of magnesium
Camara-Artigas A, et al.
Acta Crystallographica. Section F, Structural Biology Communications, 72(1), 29-35 (2016)
Jingjun Zhang et al.
Journal of Cancer, 10(12), 2822-2835 (2019-07-02)
Background: The transfusion of blood that has been stored for some time was found to be associated with transfusion-related immune modulation (TRIM) responses in cancer patients, which could result in poor clinical outcomes, such as tumor recurrence, metastasis and reduced
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