U5378
尿素
powder, BioReagent, Molecular Biology, suitable for cell culture
别名:
碳酰二胺, 碳酰胺
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关于此项目
线性分子式:
NH2CONH2
化学文摘社编号:
分子量:
60.06
UNSPSC Code:
12352111
NACRES:
NA.31
PubChem Substance ID:
EC Number:
200-315-5
Beilstein/REAXYS Number:
635724
MDL number:
Grade:
Molecular Biology
Assay:
≥98%
Technique(s):
cell culture | mammalian: suitable
产品名称
尿素, powder, BioReagent, Molecular Biology, suitable for cell culture
InChI key
XSQUKJJJFZCRTK-UHFFFAOYSA-N
InChI
1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
SMILES string
NC(N)=O
grade
Molecular Biology
product line
BioReagent
assay
≥98%
form
powder
technique(s)
cell culture | mammalian: suitable
mp
132-135 °C (lit.)
solubility
H2O: soluble 480 mg/mL, clear, colorless (8M)
density
1.335 g/mL at 25 °C (lit.)
anion traces
chloride (Cl-): ≤5 ppm
cation traces
Cu: ≤1 ppm
Fe: ≤5 ppm
Pb: ≤2 ppm
absorption
≤0.06 at 260 at 6 M
≤0.06 at 280 at 6 M
foreign activity
DNase, NICKase, RNase and protease, none detected
Quality Level
Gene Information
human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)
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Application
- 尿素已可用于蛋白质重悬缓冲液和蛋白质变性。
- 它已可用于通过含有尿素的聚丙烯酰胺凝胶分离蛋白质。
- 它被用作研究不同浓度尿素和不同pH值下未折叠蛋白的构象。
用于蛋白质变性以及作为不溶性或变性蛋白的温和增溶剂。可用于从已由 6M 盐酸胍变性的样品(如包涵体)复性蛋白质。可与盐酸胍和二硫苏糖醇 (DTT) 一起用于复性变性蛋白以恢复其天然或活性形式。
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Labeling and identification of direct kinase substrates.
Carlson SM and White FM
Science Signaling, 5, pl3-pl3 (2012)
Construction and transfection of a ribozyme targeting human caspase-3.
Cronin D et al.
Anticancer Research, 24, 425-425 (2004)
Structure and properties of native and unfolded lysing enzyme from T. harzianum: Chemical and pH denaturation.
Bey H et al.
International Journal of Biological Macromolecules, 92, 860-860 (2016)
Ziyi Yin et al.
Autophagy, 15(7), 1234-1257 (2019-02-20)
Macroautophagy/autophagy is critical for normal appressorium formation and pathogenicity of the rice blast fungus Magnaporthe oryzae, but the molecular base of autophagy linked to pathogenicity remains elusive in this or other pathogenic fungi. We found that MoHat1, a histone acetyltransferase
Gurudayal et al.
Nano letters, 15(6), 3833-3839 (2015-05-06)
Photoelectrochemical water splitting half reactions on semiconducting photoelectrodes have received much attention but efficient overall water splitting driven by a single photoelectrode has remained elusive due to stringent electronic and thermodynamic property requirements. Utilizing a tandem configuration wherein the total
相关内容
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