推荐产品
生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
MC3, monoclonal
表单
buffered aqueous solution
分子量
antigen ~65 kDa
种属反应性
human, rat, Xenopus, mouse
包装
antibody small pack of 25 μL
技术
electron microscopy: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using total cell extract from HeLa cells
同位素/亚型
IgG2b
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... U2AF2(11338)
mouse ... U2af2(22185)
rat ... U2af2(308335)
一般描述
Monoclonal Anti-U2AF65 (mouse IgG2b isotype) is derived from the MC3 hybridoma produced by the fusion of mouse myeloma cells (Ag8.653 cells) and splenocytes from BALB/c mice immunized with recombinant human U2AF65. U1, U2, U4/U6, and U5 are small nuclear riboprotein (snRNPs) that are major subunits of spliceosomes. Spliceosomes also contain non snRNPs proteins such as alternative splicing factor/splicing factor 2 (ASF/SF2), 35 kDa spliceosomal component (SC-35) and U2 snRNP auxiliary splicing factor (U2AF). U2 snRNP auxiliary factor 65 kDa (U2AF65) is a splicing factor which has three C-terminal RNA recognition motifs (RRMs) and an N-terminal arginine/serine-rich (RS) domain. This gene is mapped to human chromosome 19q13.42. Splicing factor U2AF65kDa subunit is a non-snRNP protein component of spliceosome complexes.
免疫原
recombinant human U2AF65.
应用
Monoclonal Anti-U2AF65 antibody produced in mouse has been used in:
- RNA immunoprecipitation
- immunoblotting
- chromatin immunoprecipitation (ChIP)
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemistry
- immunocytochemistry
- immunoelectron microscopy
生化/生理作用
U2 snRNP auxiliary factor 65 kDa (U2AF65)/U2AF2 helps to supply the small nuclear ribonucleoprotein (snRNP) U2, which is subunit of the spliceosome. It can induce the exclusion of alternative exons. U2AF65 primarily regulates the splicing of pre-mRNA. The serine-rich (RS) repeats at the N-terminal region act as a signalling factor for nuclear localization. (U2AF65) and (U2AF35) are components of the splicing factor U2AF that transports between the cytoplasm and the nucleus.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Gloria Barbarani et al.
Scientific reports, 7(1), 14088-14088 (2017-10-28)
The Sox6 transcription factor is crucial for terminal maturation of definitive red blood cells. Sox6-null mouse fetuses present misshapen and nucleated erythrocytes, due to impaired actin assembly and cytoskeleton stability. These defects are accompanied with a reduced survival of Sox6
Splicing of many human genes involves sites embedded within introns
Kelly S, et al.
Nucleic Acids Research, 43(9), 4721-4732 (2015)
Identification of U2AF (35)-dependent exons by RNA-Seq reveals a link between 3? splice-site organization and activity of U2AF-related proteins
Kralovicova J, et al.
Nucleic Acids Research, 43(7), 3747-3763 (2015)
Splicing inhibition of U2AF65 leads to alternative exon skipping
Cho S, et al.
Proceedings of the National Academy of Sciences of the USA, 112(32), 9926-9931 (2015)
In vitro iCLIP-based modeling uncovers how the splicing factor U2AF2 relies on regulation by cofactors
Sutandy FXR, et al.
Genome Research, 28(5), 699-713 (2018)
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