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一般描述
亚基分子量：约 90,770
由六个亚基组成，总分子量：约 544620
由六个亚基组成，总分子量：约 544620
脲酶（Ure）存在于胞质溶胶中。它在酸性 pH 下具有活性，在 28℃ 下表达。脲酶结合镍，存在于古细菌、细菌、单细胞真核生物和植物中。矮刀豆脲酶是第一个被结晶的酶，同时也是第一个被发现的含镍酶。它是一种多亚基酶，由三种蛋白质形式的91 kDa亚基组成。
应用
来自矮刀豆的脲酶已被用于：对尿样加标以测定纯尿中尿素的水解速率它还被用于研究多卤代苯并萘醌和萘醌对巴斯德芽孢杆菌和洋刀豆的抑制作用。
生化/生理作用
在洋刀豆（Jack Bean）中，脲酶促进L-刀豆氨酸转化为副刀豆氨酸。
脲酶参与嘌呤代谢和尿素循环。它水解尿素以生产氨和二氧化碳。
单位定义
一微摩尔单位是指在pH 7.0，25℃下每分钟催化尿素释放1.0 μM NH3所需的酶活性。它相当于1.0 I.U.或0.054 Sumner单位（在pH 7.0，20°C，5分钟内产生1.0 mg氨氮）
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 1
法规信息
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Armored Urease: Enzyme-Bioconjugated Poly(acrylamide) Hydrogel as a Storage and Sensing Platform
Kunduru KR, et al.
Test, 590, 143-167 (2017)
An investigation of the use of urease-antibody conjugates in enzyme immunoassays
Chandler HM, et al.
Journal of Immunological Methods, 53(2), 187-194 (1982)
G A Rosenthal et al.
Plant physiology, 76(2), 541-544 (1984-10-01)
l-Canavanine, the guanidinooxy structural analog of l-arginine, is an important nonprotein amino acid of many leguminous plants with nitrogen storage a major proported role. l-[Guanidinooxy-(14)C]canavanine, [(14)C] urea, and [(15)N]urea were injected separately into the fleshy, green cotyledons of 9-day old
Isothermal titration calorimetry to characterize enzymatic reactions
Methods in Enzymology, 567, 215-236 (2016)
Xavier Arqué et al.
ACS nano, 16(5), 7547-7558 (2022-04-30)
The increasing resistance of bacteria to existing antibiotics constitutes a major public health threat globally. Most current antibiotic treatments are hindered by poor delivery to the infection site, leading to undesired off-target effects and drug resistance development and spread. Here
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Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.
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