生物来源
human
质量水平
重组
expressed in E. coli overproducing strain
检测方案
≥95% (SDS-PAGE)
形式
solution
分子量
~32 kDa
应用
cell analysis
运输
dry ice
储存温度
−70°C
基因信息
human ... CDC34(997) , UBE2N(7334)
生化/生理作用
Cdc34 is an essential gene for viability in yeast. Cdc34 mediates the transition from G1 to S-phase of the cell cycle by degrading the S-phase cyclin/CDK inhibitor, SIC1 in yeast and p27(KIP1) in human. Cdc34 is also known to interact with the Skp1/Cdc53/F-box (SCF) ubiquitin ligase subunits Cul1 to degrade the NF-κB inhibitor IκBα in a phosphorylation-dependent manner.
Infected cell protein 0 of herpes simplex virus 1 was shown to bind UbcH3 and induce its degradation by promoting its polyubiquination. This resulted in increased expression of an UbcH3-target gene cyclin D1 and aided in further viral infection.
外形
Solution in 50 mM HEPES, 200 mM NaCl, 1 mM TCEP (pH 7.5), and 10% glycerol.
其他说明
Manufactured for Sigma by Boston Biochem. Inc.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Ryan Hagglund et al.
Proceedings of the National Academy of Sciences of the United States of America, 99(2), 631-636 (2002-01-24)
Infected cell protein 0 (ICP0) of herpes simplex virus 1, a multifunctional ring finger protein, enhances the expression of genes introduced into cells by infection or transfection, interacts with numerous cellular and viral proteins, and is associated with the degradation
Evangelia Koutelou et al.
The Journal of biological chemistry, 283(7), 3846-3853 (2007-12-14)
Notch signaling constitutes an evolutionarily conserved mechanism that mediates cell-cell interactions in various developmental processes. Numerous regulatory proteins interact with the Notch receptor and its ligands and control signaling at multiple levels. Ubiquitination and endocytosis followed by endosomal sorting of
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