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一般描述
亚基分子量：约 90,770
由六个亚基组成，总分子量：约 544620
由六个亚基组成，总分子量：约 544620
洋刀豆（直生刀豆）的尿素酶是一种含有840个氨基酸残基的单链六聚体，存在于植物、细菌、真菌、藻类和土壤中。它被视为是第一种含镍的酶，也是第一种结晶化酶。尿素酶以多种异构体存在，部分包括洋刀豆尿素酶（JBURE-I）、刀豆毒素（CNTX）和JBURE-IIB。JBURE-I比其他两种异构体更加丰富。尿素酶存在成熟的种子中。
应用
来自直生刀豆（洋刀豆）的尿素酶已用于：
- 使用从左心室（LV）和室间隔组织中提取的样本测定肌酸和磷酸肌酸
- 测定稳定尿样中尿素的浓度
- 一种标准品，以测定植物提取物的尿素酶活性
生化/生理作用
在植物中，尿素酶对于自然界中氮的生物利用度/代谢和防御机制至关重要。尿素酶是一种用于催化尿素水解的金属酶，以释放生长所需氮源—氨以及二氧化碳。他们表现内部肽介导的杀虫作用和杀菌活性。
脲酶活性已显示在暴露于光后被苯并芘抑制。这种抑制作用是由于苯并芘对脲酶′巯基的作用。
单位定义
一微摩尔单位是指在pH 7.0，25℃下每分钟催化尿素释放1.0 μmole NH3所需的酶活性。它相当于1.0 I.U.或0.054 Sumner单位（在pH 7.0，20℃下，5分钟内产生1.0 mg氨氮）
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Effect of light-activated benzpyrene on urease activity.
G C MILLS et al.
Cancer research, 13(1), 69-72 (1953-01-01)
Fernando Medina Ferrer et al.
Geobiology, 20(1), 79-97 (2021-08-03)
Modern carbonate tufa towers in the alkaline (~pH 9.5) Big Soda Lake (BSL), Nevada, exhibit rapid precipitation rates (exceeding 3 cm/year) and host diverse microbial communities. Geochemical indicators reveal that carbonate precipitation is, in part, promoted by the mixing of calcium-rich
Melissa Postal et al.
Peptides, 38(1), 22-32 (2012-08-28)
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Fernando Medina Ferrer et al.
Microbial biotechnology, 13(6), 1877-1888 (2020-07-29)
Microbial precipitation of calcium carbonate is a widespread environmental phenomenon that has diverse engineering applications, from building and soil restoration to carbon sequestration. Urease-mediated ureolysis and CO2 (de)hydration by carbonic anhydrase (CA) are known for their potential to precipitate carbonate
Jeong Hoon Lee et al.
Helicobacter, 17(6), 405-410 (2012-10-17)
Helicobacter pylori eradication is essential for metachronous gastric cancer prevention in patients undergoing endoscopic mucosectomy (EMR). This study was aimed to determine the optimal biopsy site for H. pylori detection in the atrophic remnant mucosa of EMR patients. Data were
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Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.
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