描述
wavelength 595 nm
total test time 2 min
working reagent stability 18-26°C for 2 weeks
dynamic range 50 mg/dL 0.5 g/L
质量水平
参数
18-26 °C temperature
50 μL sample volume
储存温度
2-8°C
一般描述
蛋白质测定是生化研究最常见的实验操作之一。微量总蛋白试剂盒提供了一种简单、快速、高灵敏度的方法，只需 50μ L 或更少的样品。
应用
微量总蛋白试剂盒已被用于测定:
- 通过Bradford 法测定细菌匀浆液中的总蛋白
- 通过Bradford 微量检测法测定组织提取物中的总蛋白含量
- 血清总蛋白
- 通过Bradford 法测定叶片中的可溶性蛋白质
生化/生理作用
微量总蛋白试剂盒可用于测定溶液中蛋白质的浓度。
适用性
可测定多达0.5mg/ml 的蛋白样品浓度。
原理
亮蓝G-250(考马斯亮蓝)在酸性条件下，与蛋白质反应，形成蓝色蛋白质染料复合物。该复合物在595nm处的吸光度与蛋白质含量成正比。
测试方法
比色法，终点法（Endpoint）
警示用语：
Danger
危险分类
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1
靶器官
Eyes,Central nervous system
储存分类代码
3 - Flammable liquids
闪点(°F)
132.8 °F
闪点(°C)
56 °C
法规信息
高风险级别生物产品--人源产品
危险化学品
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