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Merck
CN

TA0100

TargeTron™ 基因敲除系统

Bacterial Gene Knockout

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UNSPSC Code:
12352202

主要文件

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shipped in

wet ice

storage temp.

−20°C

General description

如需更多详细信息，请访问TargeTron™主页：www.sigma-aldrich.com/targetron。

TargeTron基因敲除系统可通过插入II型内含子为细菌基因的快速特异性破坏提供优化的试剂和实验流程。该方法利用II型内含子的逆转录能力以及一个简单的PCR步骤来重新靶向TargeTron II型内含子，以实现特异性的插入宿主基因组。该技术的实用性已被证明可用于原核基因工程、系统生物学和功能基因组学方法。

与现有的敲除方法相比，TargeTron系统非常快速、简单且强大。该过程包括将目标基因序列加载到Sigma网站（www.sigma-aldrich.com/targetronaccess）上经过充分验证的TargeTron算法、使用一个或多个输出的引物组通过PCR来突变TargeTron内含子模板、将片段连接至pACD4K-C线性载体中、转化宿主、诱导基因破坏、通过卡那霉素抗性选择（插入活化标记物）以及筛选所需的敲除结果。

TargeTron基因敲除系统已在广泛的细菌菌株中得到验证，如大肠杆菌、金黄色葡萄球菌、产气荚膜梭菌、福氏志贺菌、鼠伤寒沙门氏菌和乳酸乳球菌。该系统可经修饰而用于其他物种。

每款TargeTron试剂盒都为指定数量的设计（3 EA或10 EA）提供了访问卡和密码。该密码可使用户能够访问经过广泛验证的TargeTron算法以预测最佳的内含子插入位点，并设计用于突变内含子以插入这些位点的引物。在选择一个或多个所需插入点后，可订购所需的寡核苷酸并使用该试剂盒开始实验过程。3 EA试剂盒中的试剂足以进行3次设计及12次反应。10 EA试剂盒中的试剂足以进行10次设计及40次反应。

Application

TargeTron™基因敲除系统已用于在人单核细胞衍生的巨噬细胞中进行基因的定点突变。它也被用于在金黄色葡萄球菌中破坏核酸酶基因nuc。

Features and Benefits

靶标及永久性基因破坏
简易、简化的实验流程；最多3天内完成敲除
最小的筛选以分离突变体
>90%成功靶向插入
无细胞偶联或特异性宿主因子需要

Other Notes

如需观看TargeTron基因敲除系统动画，请访问sigma.com/targetronanimation。
系统用户手册
TargeTron访问卡（在线目标位点选择密码）
内含子PCR模板
EBS通用引物
LacZ对照引物
pACD4K-C线性载体(20 ng/μl)
JumpStart™ REDTaq® ReadyMix®
HindIII 20 U/μl
BsrGI 10 U/μl
10x 限制性酶缓冲液

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
TargeTron is a trademark of InGex, LLC

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

新产品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLBS8006
SLBS8005
SLBS3309V
SLBS3308V
SLBS2445V

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如果您需要特殊版本，可通过批号或批次号查找具体证书。

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  1. How stable is the knockout using TargeTron, Product TA0100 after several generations?

    Data in Lactococcus lactis mutants passaged for 80 generations illustrated that 100% of the mutants were stable.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. How can I determine possible targets with the Targetron® algorithim?  How do I get my primer designs?

    Please click here to submit your genomic sequence of interest to Technical Service.  We will let you know your available target regions according to the algorithim via email reply.If you are ready to obtain your primer designs, please provide your genomic sequence and your access code from your Targetron® kit in the email and we will provide your designs to you via email reply.

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Sanath Kumar et al.
Archives of microbiology, 193(3), 201-208 (2010-12-25)
A putative mannitol operon of the phosphoenolpyruvate phosphotransferase (PTS) type was cloned from Vibrio cholerae O395, and its activity was studied in Escherichia coli. The 3.9-kb operon comprising three genes is organized as mtlADR. Based on the sequence analysis, these
Staphylococcus aureus ?-Toxin Mutants Are Defective in Biofilm Ligase and Sphingomyelinase Activity, and Causation of Infective Endocarditis and Sepsis.
Herrera A
Biochemistry, 55(17), 2510-2517 (2016)
M Chelsea Lane et al.
Journal of bacteriology, 191(5), 1382-1392 (2008-12-31)
MR/P fimbriae of uropathogenic Proteus mirabilis undergo invertible element-mediated phase variation whereby an individual bacterium switches between expressing fimbriae (phase ON) and not expressing fimbriae (phase OFF). Under different conditions, the percentage of fimbriate bacteria within a population varies and
Greta R Nielubowicz et al.
Infection and immunity, 76(9), 4222-4231 (2008-07-16)
Proteus mirabilis, a gram-negative bacterium, is a frequent cause of complicated urinary tract infections in those with functional or anatomical abnormalities or those subject to long-term catheterization. To systematically identify surface-exposed antigens as potential vaccine candidates, proteins in the outer
Tao Wang et al.
Frontiers in cellular and infection microbiology, 8, 192-192 (2018-06-26)
Lipid A is an essential basal component of lipopolysaccharide of most Gram-negative bacteria. Inhibitors targeting LpxC, a conserved enzyme in lipid A biosynthesis, are antibiotic candidates against Gram-negative pathogens. Here we report the characterization of the role of lipid A
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