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安全信息

T8778

Sigma-Aldrich

海藻糖酶 来源于猪肾脏

buffered aqueous glycerol solution, ≥1.0 units/mg protein

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别名:
αα-海藻糖-葡萄糖水解酶
CAS号:
9025-52-9
EC 号:
3.2.1.28 (BRENDA, IUBMB)
MDL编号:
MFCD00132462
UNSPSC代码:
12352204
NACRES:
NA.54

形式

buffered aqueous glycerol solution

质量水平

200

比活

≥1.0 units/mg protein

浓度

0.5-10.0 mg/mL protein (BCA)

储存温度

−20°C

相关类别

一般描述

海藻糖是大多数昆虫体内的主要血淋巴糖，是入侵病原真菌的主要靶标。
海藻糖酶(Trehalase)是一种糖苷酶，具有多种形式，存在于所有组织中。属于碳水化合物活性酶(CAZy)类中的糖苷水解酶37(GH37)家族成员。

应用

海藻糖已用于：
  • 评价葡萄霜霉病菌感染葡萄藤叶的糖代谢变化
  • 研究海藻糖-6-磷酸介导的生长停滞
  • 定量分析干酪乳杆菌Lactobacillus casei细胞内的海藻糖

生化/生理作用

海藻糖酶可催化水解一分子海藻糖生成两分子葡萄糖。它介导飞行代谢、蜕皮诱导的几丁质合成和低温耐受。

单位定义

一个单位将在37°C、pH 5.7 下每分钟将 1.0μmol 海藻糖转化为 2.0 μg葡萄糖（pH 7.5 下测定释放的葡萄糖）。

外形

溶液溶于含 1%Triton ™ X-100 和 25 mM 磷酸钾的 50% 甘油，pH 6.5

法律信息

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How is Product T8778, Trehalase from porcine kidney prepared?

    The actual method of preparation utilized by Sigma for Product T8778, Trehalase is confidential, but does include ion exchange and size exclusion chromatography. A method of preparation for Trehalase has been described in Archives of Biochemistry and Biophysics, 255, 168 - 175 (1987).

  6. What is the molecular weight of Product T8778, Trehalase from porcine kidney?

    The molecular weight of Product T8778, Trehalase from porcine kidney has an estimated molecular weight of 80,000 daltons upon SDS-PAGE, as described by Yoshitaka Yoneyama in Archives of Biochemistry and Biophysics, 255, 168 - 175 (1987). This molecular weight is similar to that reported for mouse Trehalase which has an estimated molecular weight by gel filtration on Sephadex G-200 of 73,000 daltons, as described in Anais Da Academia Brasileira De Ciencias, 51, 151 - 158 (1979). 

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Shari Dhaene et al.
Journal of enzyme inhibition and medicinal chemistry, 35(1), 1964-1989 (2020-11-10)
Although trehalose has recently gained interest because of its pharmaceutical potential, its clinical use is hampered due to its low bioavailability. Hence, hydrolysis-resistant trehalose analogues retaining biological activity could be of interest. In this study, 34 4- and 6-O-substituted trehalose
Growth arrest by trehalose-6-phosphate: an astonishing case of primary metabolite control over growth by way of the SnRK1 signaling pathway
Delatte, T., et al.
Annual review of plant physiology and plant molecular biology, 157, 160-174 (2011)
Davide Bini et al.
Beilstein journal of organic chemistry, 8, 514-521 (2012-04-18)
A small set of nojirimycin- and pyrrolidine-based iminosugar derivatives has been synthesized and evaluated as potential inhibitors of porcine and insect trehalases. Compounds 12, 13 and 20 proved to be active against both insect and porcine trehalases with selectivity towards
Jie Chen et al.
PloS one, 5(4), e10133-e10133 (2010-04-21)
Trehalase, an enzyme that hydrolyzes trehalose to yield two glucose molecules, plays a pivotal role in various physiological processes. In recent years, trehalase proteins have been purified from several insect species and are divided into soluble (Tre-1) and membrane-bound (Tre-2)
Trehalose-hydrolysing enzymes of Metarhizium anisopliae and their role in pathogenesis of the tobacco hornworm, Manduca sexta
Xia Y, Clarkson JM, Charnley AK.
J. Invert. Path., 80(3), 139-147 (2012)
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