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安全信息

T8778

Sigma-Aldrich

海藻糖酶 来源于猪肾脏

buffered aqueous glycerol solution, ≥1.0 units/mg protein

别名:

αα-海藻糖-葡萄糖水解酶

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About This Item

CAS号:
9025-52-9
EC 号:
3.2.1.28 (BRENDA, IUBMB)
MDL编号:
MFCD00132462
UNSPSC代码:
12352204
NACRES:
NA.54

表单

buffered aqueous glycerol solution

质量水平

200

比活

≥1.0 units/mg protein

浓度

0.5-10.0 mg/mL protein (BCA)

储存温度

−20°C

相关类别

一般描述

海藻糖是大多数昆虫体内的主要血淋巴糖，是入侵病原真菌的主要靶标。
海藻糖酶(Trehalase)是一种糖苷酶，具有多种形式，存在于所有组织中。属于碳水化合物活性酶(CAZy)类中的糖苷水解酶37(GH37)家族成员。

应用

海藻糖已用于：
  • 评价葡萄霜霉病菌感染葡萄藤叶的糖代谢变化
  • 研究海藻糖-6-磷酸介导的生长停滞
  • 定量分析干酪乳杆菌Lactobacillus casei细胞内的海藻糖

生化/生理作用

海藻糖酶可催化水解一分子海藻糖生成两分子葡萄糖。它介导飞行代谢、蜕皮诱导的几丁质合成和低温耐受。

单位定义

一个单位将在37°C、pH 5.7 下每分钟将 1.0μmol 海藻糖转化为 2.0 μg葡萄糖（pH 7.5 下测定释放的葡萄糖）。

外形

溶液溶于含 1%Triton ™ X-100 和 25 mM 磷酸钾的 50% 甘油，pH 6.5

法律信息

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物源性产品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
0000280313
0000352066
0000352227
0000280313
0000352066

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How is Product T8778, Trehalase from porcine kidney prepared?

    The actual method of preparation utilized by Sigma for Product T8778, Trehalase is confidential, but does include ion exchange and size exclusion chromatography. A method of preparation for Trehalase has been described in Archives of Biochemistry and Biophysics, 255, 168 - 175 (1987).

  6. What is the molecular weight of Product T8778, Trehalase from porcine kidney?

    The molecular weight of Product T8778, Trehalase from porcine kidney has an estimated molecular weight of 80,000 daltons upon SDS-PAGE, as described by Yoshitaka Yoneyama in Archives of Biochemistry and Biophysics, 255, 168 - 175 (1987). This molecular weight is similar to that reported for mouse Trehalase which has an estimated molecular weight by gel filtration on Sephadex G-200 of 73,000 daltons, as described in Anais Da Academia Brasileira De Ciencias, 51, 151 - 158 (1979). 

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Growth arrest by trehalose-6-phosphate: an astonishing case of primary metabolite control over growth by way of the SnRK1 signaling pathway
Delatte, T., et al.
Annual review of plant physiology and plant molecular biology, 157, 160-174 (2011)
Shari Dhaene et al.
Journal of enzyme inhibition and medicinal chemistry, 35(1), 1964-1989 (2020-11-10)
Although trehalose has recently gained interest because of its pharmaceutical potential, its clinical use is hampered due to its low bioavailability. Hence, hydrolysis-resistant trehalose analogues retaining biological activity could be of interest. In this study, 34 4- and 6-O-substituted trehalose
Dimitri A Skandalis et al.
Physiological and biochemical zoology : PBZ, 85(6), 657-670 (2012-10-27)
Although intraspecific variation in metabolic rate is associated with variation in body size, similarly sized individuals nonetheless vary greatly. At similar masses, hovering bumblebee workers (Bombus impatiens) can differ in metabolic rate up to twofold. We examined how such interindividual
Choowong Auesukaree et al.
Journal of bioscience and bioengineering, 114(2), 144-149 (2012-05-15)
For industrial applications, fermentation of ethanol at high temperature offers advantages such as reduction in cooling costs, reduced risk of microbial contamination and higher efficiency of fermentation processes including saccharification and continuous ethanol stripping. Three thermotolerant Saccharomyces cerevisiae isolates (C3723
Yu Sasano et al.
Journal of bioscience and bioengineering, 113(5), 592-595 (2012-01-28)
Freeze tolerance is a necessary characteristic for industrial baker's yeast because frozen-dough baking is one of the key technologies for supplying oven-fresh bakery products to consumers. Both proline and trehalose are known to function as cryoprotectants in yeast cells. In
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