T7902
嗜热菌蛋白酶 from Geobacillus stearothermophilus
powder, BioReagent, 30-350 units/mg protein (E1%/280), suitable for cell culture
别名:
嗜热菌蛋白酶, 蛋白酶 来源于六甲嗜热溶蛋白芽孢杆菌
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.75
EC Number:
232-973-4
MDL number:
Specific activity:
30-350 units/mg protein (E1%/280)
Biological source:
Geobacillus stearothermophilus
产品名称
嗜热菌蛋白酶 from Geobacillus stearothermophilus, powder, BioReagent, 30-350 units/mg protein (E1%/280), suitable for cell culture
biological source
Geobacillus stearothermophilus
product line
BioReagent
form
powder
specific activity
30-350 units/mg protein (E1%/280)
mol wt
34.6 kDa
purified by
crystallization
technique(s)
cell culture | mammalian: suitable
single cell analysis: suitable
storage temp.
−20°C
Quality Level
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Application
一种含有四个钙离子的热稳定性（嗜热性）细胞外金属内肽酶。辅助因子是锌和钙。水解疏水性氨基酸残基N-末端的蛋白质键。最适pH为8.0，活性的最适温度为70℃。在pH 5-9.5范围内非常稳定。嗜热菌蛋白酶具有较低的切割特异性，因此，它能产生许多适合测序的短片段。优先切割顺序： X-切割-Y-Z，其中X = 任何氨基酸；Y = Leu，Phe，Ile，Val，Met，Ala；Z是除Pro以外的任何氨基酸。切割Leu的N末端优先于切割Phe的N末端，它们优于其他氨基酸。常用于限制性蛋白水解以绘制肽图谱以及蛋白质结构和构象变化研究。
Other Notes
在37℃、pH7.5下，一个单位每分钟将水解酪蛋白产生相当于1.0 μmol （181 μg）7.5克酪氨酸（福林-乔卡尔特试剂显色）。
Physical form
含钙和乙酸钠缓冲盐的冻干粉
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
此项目有
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The biophysical stability is an important parameter for protein activity both in vivo and in vitro. Here we propose a method to analyse thermal melting of protein domains in lysates: Fast parallel proteolysis (FASTpp). Combining unfolding by a temperature gradient
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Yuichi Kawasaki et al.
Journal of biochemistry, 153(1), 85-92 (2012-10-23)
The thermolysin variant G8C/N60C/S65P in which the triple mutation in the N-terminal domain, Gly8→Cys/Asn60→Cys/Ser65→Pro, is undertaken increases stability [Yasukawa, K. and Inouye, K. (2007) Improving the activity and stability of thermolysin by site-directed mutagenesis. Biochim. Biophys. Acta 1774, 1281-1288] and
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