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Merck
CN
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文件

安全信息

T7659

Sigma-Aldrich

胰蛋白酶抑制剂,确定的(1X)溶液

Animal component free, BioReagent, suitable for cell culture

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CAS号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.75

质量水平

无菌性

sterile-filtered

产品线

BioReagent

形式

solution

技术

cell culture | mammalian: suitable

运输

dry ice

储存温度

−20°C

应用

用于中和胰蛋白酶/EDTA溶液的作用。
设计用于角质形成细胞传代培养的本品含有Kunitz型大豆胰蛋白酶抑制剂(SBTI),可抑制丝氨酸蛋白酶(如胰蛋白酶和类胰蛋白酶)的催化活性。 它用于细胞培养以终止胰蛋白酶的作用,胰蛋白酶用于在传代过程中从基质中释放细胞。 它也可能用于其他抗丝氨酸蛋白酶研究。

外形

溶于98.7% DPBS和1.3%大豆胰蛋白酶抑制剂中。

重悬

以1:1 TID:胰蛋白酶/EDTA的最小体积比使用。

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Resp. Sens. 1 - Skin Sens. 1

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

动植物源性产品

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Sarra Djemil et al.
Cellular and molecular neurobiology, 41(8), 1787-1799 (2020-08-30)
Septal innervation of basal forebrain cholinergic neurons to the hippocampus is critical for normal learning and memory and is severely degenerated in Alzheimer's disease. To understand the molecular events underlying physiological cholinergic synaptogenesis and remodeling, as well as pathological loss
Maurizio Trovato et al.
Biochemical and biophysical research communications, 302(2), 311-315 (2003-02-27)
The design of minimal units required for enzyme inhibition is a major field of interest in structural biology and biotechnology. The successful design of the cyclic dodecapeptide corresponding to the Phe17-Val28 reactive site amino acid sequence of the low-molecular-mass trypsin
C L Dumke et al.
Journal of applied physiology (Bethesda, Md. : 1985), 92(2), 657-664 (2002-01-18)
Serum proteins [molecular weight (MW) > 10,000] are essential for increased insulin-stimulated glucose transport after in vitro muscle contractions. We investigated the role of the kallikrein-kininogen system, including bradykinin, which is derived from kallikrein (MW > 10,000)-catalyzed degradation of serum
I B Svendsen et al.
Carlsberg research communications, 54(6), 231-239 (1989-01-01)
A trypsin inhibitor with a Km of 5 x 10(-5) M has been isolated from kohlrabi (Brassica napus var. rapifera). Subtilisin DY is inhibited only weakly and chymotrypsin not at all. The inhibitor is closely related to napin as determined

实验方案

天然胰蛋白酶抑制剂也称为丝氨酸蛋白酶抑制剂(serpins),是最大和最多样化的蛋白酶抑制剂家族。Serpins通过抑制体内丝氨酸蛋白酶来控制蛋白质的活化和分解代谢。

Natural trypsin inhibitors (serpins) regulate protein activation and catabolism by inhibiting serine proteases in vivo.

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