推荐产品
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen 80-100 kDa
种属反应性
human
增强验证
recombinant expression
Learn more about Antibody Enhanced Validation
浓度
~1 mg/mL
技术
immunoprecipitation (IP): 10-20 μg using TGN46 from human HepG2 cell lysates.
indirect immunofluorescence: 5-10 μg/mL using human A549 cells
western blot (chemiluminescent): 0.25-0.5 μg/mL using extracts of human HEK-293T cells expressing recombinant human TGN46
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... TGOLN2(10618)
一般描述
TGN46(反式高尔基体网络蛋白,46 kDa)是大鼠TGN38的人类同源物,是反式高尔基体网络(TGN)的固有整合膜蛋白,其可在TGN和质膜之间进行组成性循环,并通过内体返回。TGN是蛋白质分泌途径、脂质和膜运输的的主要分选细胞器。TGN46是一种高度糖基化的蛋白质,可能参与调节往返TGN的膜运输。TGN46包含信号肽、内腔结构域、跨膜结构域和胞质结构域。跨膜区域和胞质尾巴分别包含保留和回收信号,可用于TGN的定位。TGN46在细胞中广泛表达。
抗TGN46可识别人反式高尔基体网络蛋白2(TGN46)。通过免疫印迹对TGN46条带的检测可被免疫肽特异性抑制。
免疫原
对应于人TGN46的氨基酸残基426-437的合成肽,N-末端添加有半胱氨酸,并与KLH偶联。与大鼠中的相应序列相同,与人的相应序列相差2个氨基酸。
应用
兔多克隆抗TGN46抗体可用于标记反式高尔基体网络蛋白2,并可通过免疫细胞化学和免疫组化(IHC)技术(例如免疫印迹、免疫沉淀和免疫荧光)进行检测和定量。它可作为探针,对反式高尔基网络蛋白2[作为反式高尔基网络(TGN)的膜转运调节成分]的存在和作用进行确定。抗TGN46可用作TGN标记。
外形
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途,或人类或动物任何类型的消费或使用。
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Andrea N Dietz et al.
Journal of virology, 92(1) (2017-10-20)
The human cytomegalovirus (HCMV) tegument protein pUL71 is required for efficient secondary envelopment and accumulates at the Golgi compartment-derived viral assembly complex (vAC) during infection. Analysis of various C-terminally truncated pUL71 proteins fused to enhanced green fluorescent protein (eGFP) identified
Yong Yu et al.
The Biochemical journal, 434(3), 383-390 (2011-01-22)
Peptide hormones and neuropeptides are packaged and stored in a specialized intracellular organelle called the dense core vesicle. It remains elusive how peptide cargoes are correctly sorted. In the present study, we show that a highly conserved Golgi-localized protein named
Jiehua Li et al.
Autophagy, 13(2), 345-358 (2016-11-23)
The membrane source for autophagosome biogenesis is an unsolved mystery in the study of autophagy. ATG16L1 forms a complex with ATG12-ATG5 (the ATG16L1 complex). The ATG16L1 complex is recruited to autophagic membranes to convert MAP1LC3B-I to MAP1LC3B-II. The ATG16L1 complex
Shuaizhi Li et al.
PloS one, 14(11), e0225496-e0225496 (2019-11-20)
Human papillomavirus (HPV) is the most common sexually transmitted pathogen in the United States, causing 99% of cervical cancers and 5% of all human cancers worldwide. HPV infection requires transport of the viral genome (vDNA) into the nucleus of basal
Arnold Hayer et al.
Traffic (Copenhagen, Denmark), 11(3), 361-382 (2010-01-15)
We analyzed the assembly of caveolae in CV1 cells by following the fate of newly synthesized caveolin-1 (CAV1), caveolin-2 and polymerase I and transcript release factor (PTRF)/cavin-1 biochemically and using live-cell imaging. Immediately after synthesis in the endoplasmic reticulum (ER)
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