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一般描述
冻干粉末，含有蔗糖、氯化钠和Tris。
凝血酶是止血过程中的最终凝血蛋白酶，可同时促进促凝血和抗凝血作用。
应用
凝血酶被用于重组融合蛋白的位点特异性切割，这些重组融合蛋白具有可接近的凝血酶识别位点以除去亲和标签。凝血酶已应用于一项研究，以评估用于小尺寸血小板微粒的流式细胞术的创新方法。
生化/生理作用
选择性裂解纤维蛋白原中 Arg-Gly 键形成纤维蛋白和纤维蛋白肽 A 和 B 的丝氨酸蛋白酶。
单位定义
活性以NIH单位表示，它是通过与NIH凝血酶参考标准品直接对比而获得的
分析说明
NIH测定程序根据Biggs改进方法，使用0.2ml稀释血浆（与生理盐水1:1）作为底物以及0.1ml凝血酶样本（在1%缓冲白蛋白溶液中稳定）。仅在15-25 秒的凝血时间内测定凝血酶浓度。
其他说明
请访问www.sigma-aldrich.com/enzymeexplorer查看有关凝血酶的更多信息。
免责声明
仅供研究使用。本品在法国的科研应用受到监管，包括用于进口和出口活动（法国《公共卫生法典》Public Health Code Article L 1211-1 paragraph 2）。如《公共卫生法典》所述，购买者（即最终用户）需要从法国研究部(France Ministry of Research)获得进口授权。订购此产品即代表您已确认获得合法的进口授权。
法规信息
高风险级别生物产品--人源产品
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Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.
凝血酶因子IIa是一种内切丝氨酸蛋白酶，可选择性地裂解纤维蛋白原的Arg -Gly键，形成纤维蛋白并释放纤维蛋白肽A和B。
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