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Merck
CN

T7193

Sigma-Aldrich

®缓冲剂

BioPerformance Certified, pH 7.0, average Mw 154.8

别名:

Tris 晶体

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About This Item

UNSPSC代码:
12161700
NACRES:
NA.25

等级

BioPerformance Certified

质量水平

Agency

suitable for EPA 537.1

形式

crystalline powder

分子量

average Mw 154.8

技术

cell culture | mammalian: suitable

杂质

DNase, RNase, Protease, NICKase, none detected
endotoxin, tested
total aerobic microbial count, tested
≤0.05 A290
≤0.5% water (Karl Fischer)
≤5 ppm Heavy metals (as Pb)

pH值(酸碱度)

7.00 (25 °C, 0.1 M)(lit.)
7.0

有效pH范围

7.0-9.0

溶解性

water: 0.667 g/mL, clear, colorless

应用

diagnostic assay manufacturing

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应用

Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
该产品经细胞培养测试。它还经过了低内毒素水平检测并分析了是否已去除核酸酶和蛋白酶。

其他说明

This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer.
某些pH电极不能使用Tris缓冲液提供准确的结果。

制备说明

Trizma溶液可高压灭菌。

法律信息

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves


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