T7039
转化生长因子-β1 人
≥98% (SDS-PAGE), recombinant, expressed in CHO cells, powder, suitable for cell culture
别名:
hTGF-β1, TGF-β1
产品名称
转化生长因子-β1 人, TGF-β1, recombinant, expressed in CHO cells, powder, suitable for cell culture
biological source
human
recombinant
expressed in CHO cells
assay
≥98% (SDS-PAGE)
form
powder
potency
≤0.05 ng/mL ED50
quality
endotoxin tested
mol wt
protein 25 kDa
packaging
pkg of 2 μg
pkg of 50 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
<1 EU/μg
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... TGFB1(7040)
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Analysis Note
通过在培养物中抑制小鼠HT-2细胞的小鼠IL-4依赖性增殖，测定TGF-β1的生物活性。
Application
人转化生长因子-β1已用于：
- 报告基因检测。
- 体外转染。
- 通过基因阵列分析研究转化生长因子-β1 (TGF-β1)对肾细胞的影响。
- 在微血管内皮细胞中，研究TGF-β1与金属蛋白酶-1组织抑制剂的剪切应力依赖性上调的联系。
- 研究TGF-β1和肌成纤维细胞形成之间的相关作用。
Biochem/physiol Actions
TGF-β1是一种多功能肽，可影响多种细胞的增殖、组织生长、分化等功能。TGF-β1通过参与脂肪生成、肌肉生成、软骨生成、骨生成、上皮细胞分化和免疫细胞功能，在分化中发挥基础性作用。所有细胞都具有特异性TGF-β1受体。转化和非肿瘤组织均释放转化生长因子。TGF-β1作用机制的多态性在于，它既可刺激也可抑制细胞增殖。TGF-β1与结直肠癌的发展和转移有关。
转化生长因子-β1 (TGF-β1)由多种细胞合成，但据报道，其含量在哺乳动物血小板中最丰富，约为TGF-β2浓度的四倍。
General description
Tgfb1（转化生长因子-β1，人）是由位于人染色体19q13.2的基因编码的25kDa肽。TGF-β1多肽由前体裂解而来。前体由疏水信号序列、前体区和成熟肽组成。在四碱基切割位点断开前体，获得由羧基端112个氨基酸组成的单体。Tgfb1的生物活性形式为二硫键连接的同二聚体。在哺乳动物中，已发现三种TGF-β亚型：TGF-β1、TGF-β2和TGF-β3。
Physical form
0.2 μm过滤的水溶液冻干制成。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
此项目有
Matrix metalloproteinase inhibition delays wound healing and blocks latent transforming growth factor-β1 promoted myofibroblast formation and function
Mirastschijski U, et al.
Wound Repair and Regeneration, 18(2), 223-234 (2010)
Interplay between TGF-β signaling and receptor tyrosine kinases in tumor development.
Shi Q and Chen YG
Science China: Life Sciences, 60(10), 1133-1141 (2017)
Tibor Kempf et al.
Clinical chemistry, 53(2), 284-291 (2006-12-23)
Growth-differentiation factor 15 (GDF15) is a member of the transforming growth factor beta (TGF-beta) cytokine superfamily. There has been increasing interest in using circulating GDF15 as a biomarker in patients, for example those with cardiovascular disease. We developed an IRMA
Differential Effects of Transforming Growth Factor-β1 on Cellular Proliferation in the Developing Prostate
Tomlinson DC, et al.
Endocrinology, 145, 4292-4300 (2004)
Regulation of α-Smooth Muscle Actin Expression in Granulation Tissue Myofibroblasts Is Dependent on the Intronic CArG Element and the Transforming Growth Factor-β1 Control Element
Tomasek JJ, et al.
The American Journal of Pathology, 166, 1343-1351 (2005)
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