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产品名称
N-(对甲苯磺酰基)-甘氨酸-脯氨酸-赖氨酸-4-硝基苯胺 乙酸盐, plasmin substrate
质量水平
方案
≥98% (TLC)
表单
powder
溶解性
ethanol: 50 mg/mL, colorless to yellow
储存温度
−20°C
SMILES字符串
CC(O)=O.Cc1ccc(cc1)S(=O)(=O)NCC(=O)N2CCC[C@H]2C(=O)N[C@@H](CCCCN)C(=O)Nc3ccc(cc3)[N+]([O-])=O
InChI
1S/C26H34N6O7S.C2H4O2/c1-18-7-13-21(14-8-18)40(38,39)28-17-24(33)31-16-4-6-23(31)26(35)30-22(5-2-3-15-27)25(34)29-19-9-11-20(12-10-19)32(36)37;1-2(3)4/h7-14,22-23,28H,2-6,15-17,27H2,1H3,(H,29,34)(H,30,35);1H3,(H,3,4)/t22-,23-;/m0./s1
InChI key
KPGSNEYSJPIZCW-SJEIDVEUSA-N
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应用
N-对甲苯磺酰基-甘氨酰-脯氨酰-赖氨酰-4-硝基苯胺乙酸盐已被用于：
- 作为aprE2、aprE5-41的酰胺水解活性检测的底物
- 作为丝氨酸蛋白酶和纤维蛋白原水解测定的底物
- 作为纤溶酶测定的底物
生化/生理作用
N-对甲苯磺酰基-甘氨酰-脯氨酰-赖氨酰-4-硝基苯胺乙酸盐是纤溶酶测定、丝氨酸蛋白酶活性和纤维蛋白原水解测定的显色底物。
底物
纤溶酶的发色底物。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Overexpression of aprE2, a Fibrinolytic Enzyme Gene from Bacillus subtilis CH3-5, in Escherichia coli and the Properties of AprE2.
Jeong SJ, et al.
Journal of microbiology and biotechnology, 24(7), 969-978 (2014)
Min Wang et al.
International journal of biological macromolecules, 146, 781-789 (2019-11-16)
The heterologous nature of SAK, a thrombolytic drug, elicits high titers of neutralizing antibodies, which limits its clinical use. Here, we aim to establish a SAK mutant with equivalent activity to the wild type but reduced antigenicity, which may allow
Streptococcus pneumoniae choline-binding protein E interaction with plasminogen/plasmin stimulates migration across the extracellular matrix.
Attali C, et al.
Infection and Immunity, 76(2), 466-476 (2008)
A bi-functional anti-thrombosis protein containing both direct-acting fibrin (ogen) olytic and plasminogen-activating activities
Yang H, et al.
PLoS ONE, 6(3), e17519-e17519 (2011)
Michał Śmiga et al.
BMC microbiology, 19(1), 127-127 (2019-06-13)
Porphyromonas gingivalis is considered a keystone pathogen responsible for chronic periodontitis. Although several virulence factors produced by this bacterium are quite well characterized, very little is known about regulatory mechanisms that allow different strains of P. gingivalis to efficiently survive
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