T5816
三(羟甲基)甲基甘氨酸
BioPerformance Certified, suitable for cell culture, ≥99% (titration)
别名:
2-[[1,3-二羟基-2-(羟甲基)丙-2-基]氨基]乙酸, N-[三（羟甲基）甲基]甘氨酸, N-三(羟甲基)甲基甘氨酸
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关于此项目
线性分子式:
(HOCH2)3CNHCH2CO2H
化学文摘社编号:
分子量:
179.17
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
EC Number:
227-193-6
Beilstein/REAXYS Number:
1937804
MDL number:
产品名称
三(羟甲基)甲基甘氨酸, BioPerformance Certified, suitable for cell culture, ≥99% (titration)
InChI key
SEQKRHFRPICQDD-UHFFFAOYSA-N
InChI
1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
SMILES string
OCC(CO)(CO)NCC(O)=O
grade
BioPerformance Certified
assay
≥99% (titration)
form
crystalline powder
storage condition
dry at room temperature
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin and total aerobic microbial count, tested
color
white
useful pH range
7.4-8.8
pKa (25 °C)
8.1
mp
187 °C
cation traces
heavy metals (as Pb): ≤5 ppm
absorption
≤0.02 at 290 at 20%
application(s)
diagnostic assay manufacturing
general analytical
life science and biopharma
sample preparation
Quality Level
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Application
三（羟甲基）甲基甘氨酸作为一种多功能缓冲剂，适用于在低百分比的丙烯酰胺凝胶中分离低分子量的蛋白质，已经证明使用萤火虫荧光素酶进行ATP测定的有效性，并在研究辐射引起的膜损伤时表现出清除羟自由基的潜力。此外，它在分离和纯化参与特定mRNA翻译并含有特定肽-tRNA分子的核糖体中发挥了作用。.三（羟甲基）甲基甘氨酸还被用于合成RNA寡核苷酸和制备高密度DNA微阵列，从而能够通过光刻术法探索核酸·配体相互作用和文库构建的机制。
用于分离低分子量的肽的缓冲剂组分。
Features and Benefits
- 适用作为缓冲剂组分，用于电泳和蛋白质分离
- 有效缓冲pH 7.4-8.8（25 °C），pKa为8.1（25 °C）
- 测试内毒素和总有氧微生物计数
- 测试以确认低水平的重金属污染，确保各种应用的适用性
General description
三（羟甲基）甲基甘氨酸是一种带电离子的生物缓冲剂，通常用于酶测定、电泳和细胞培养研究。与甘氨酸相比，它具有更高的负电荷，这使得它能够更快地迁移。三（羟甲基）甲基甘氨酸′的高离子强度能够引起更多的离子迁移和更少的蛋白质迁移，从而在低丙烯酰胺凝胶中分离低分子量蛋白质。已经观察到，通过电泳，三（羟甲基）甲基甘氨酸在1至100 kDa蛋白质分离中的有效性。根据一项研究，在使用萤火虫荧光素酶进行ATP测定时，25 mmol/L的三（羟甲基）甲基甘氨酸缓冲液是经过测试的其他十种缓冲液中表现最有效。此外，三（羟甲基）甲基甘氨酸还可用于毛细管区带电泳、高效液相色谱和离子交换色谱。
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Isolation of translating ribosomes containing peptidyl-tRNAs for functional and structural analyses.
Nitin Shirole et al.
Journal of visualized experiments : JoVE, (48)(48), doi:10-doi:10 (2011-03-16)
Recently, structural and biochemical studies have detailed many of the molecular events that occur in the ribosome during inhibition of protein synthesis by antibiotics and during nascent polypeptide synthesis. Some of these antibiotics, and regulatory nascent polypeptides mostly in the
Yi Zhou et al.
Nature, 607(7919), 527-533 (2022-07-07)
Immature dentate granule cells (imGCs) arising from adult hippocampal neurogenesis contribute to plasticity and unique brain functions in rodents1,2 and are dysregulated in multiple human neurological disorders3-5. Little is known about the molecular characteristics of adult human hippocampal imGCs, and
Arpita Gantayet et al.
Biofouling, 29(1), 77-85 (2012-12-06)
The freshwater zebra mussel (Dreissena polymorpha) is a notorious biofouling organism. It adheres to a variety of substrata underwater by means of a proteinaceous structure called the byssus, which consists of a number of threads with adhesive plaques at the
Thierry Rabilloud
Journal of proteomics, 73(8), 1562-1572 (2010-04-17)
Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.
Christian Nilsson et al.
Electrophoresis, 31(3), 459-464 (2010-02-02)
Totally porous lipid-based liquid crystalline nanoparticles were used as pseudostationary phase for capillary electroseparation with LIF detection of proteins at physiological conditions using unmodified cyclic olefin copolymer capillaries (Topas, 6.7 cm effective length). In the absence of nanoparticles, i.e. in
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