T5772
凝血酶 来源于大鼠血浆
lyophilized powder, ≥1,000 units/mg protein (biuret)
Analysis Note
NIH 试验程序使用 0.2ml 稀释血浆（与生理盐水 1:1）作为底物，0.1根据改进的 Biggs 方法，ml 凝血酶样本（在 1% 缓冲白蛋白溶液中稳定）。仅使用 15-25 秒范围内的凝血时间测定凝血酶浓度。
Application
凝血酶用于站点融合蛋白的位点特异性裂解，重组融合蛋白含有可进入的凝血酶识别站点，用于去除亲和标签。
凝血酶用于站点融合蛋白的位点特异性裂解，重组融合蛋白含有可进入的凝血酶识别站点，用于去除亲和标签。凝血酶已用于研究马富血小板血浆的活化。
Biochem/physiol Actions
选择性裂解纤维蛋白原中 Arg-Gly 键形成纤维蛋白和纤维蛋白肽 A 和 B 的丝氨酸蛋白酶。
General description
凝血酶是止血方面的最终凝血蛋白酶，促进促凝血和抗凝作用。
Other Notes
查看更多关于 凝血酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer。
通过与 NIH 凝血酶参比标准品（批次 K）直接比较，以 NIH 单位表示活性。
Physical form
从生理盐水柠檬酸钠缓冲液 (pH 6.5) 冻干
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
此项目有
Ting Yang et al.
Journal of neuroinflammation, 19(1), 120-120 (2022-05-28)
The danger-associated molecular patterns (DAMPs) are critical contributors to the progressive neuropathology and thereafter affect the functional outcomes following spinal cord injury (SCI). Up to now, the regulatory mechanisms on their inducible production from the living cells remain elusive, aside
Jamie A Textor et al.
Veterinary surgery : VS, 41(7), 784-794 (2012-06-30)
To investigate and compare clinically relevant Platelet-rich plasma (PRP) activation methods. Experimental. PRP was prepared from 6 equine subjects. Activation of the PRP was performed by 4 methods (autologous thrombin, bovine thrombin, calcium chloride (CaCl(2) ), or freeze-thaw). The resultant
Xiaojun Chen et al.
Cell death discovery, 8(1), 189-189 (2022-04-12)
Spinal cord injury (SCI) will result in the significant elevation of thrombin production at lesion site via either breakage of blood-spinal cord barrier or upregulated expression within nerve cells. Thrombin-induced activation of the protease activated receptors (PARs) evokes various pathological
Response.
Sarah T Garber et al.
Journal of neurosurgery, 118(2), 485-485 (2013-03-16)
The reply.
Peter R Kowey et al.
The American journal of medicine, 126(4), e23-e23 (2013-03-20)
商品
Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.
凝血酶因子IIa是一种内切丝氨酸蛋白酶，可选择性地裂解纤维蛋白原的Arg -Gly键，形成纤维蛋白并释放纤维蛋白肽A和B。
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