一般描述
凝血酶是止血方面的最终凝血蛋白酶，促进促凝血和抗凝作用。
应用
凝血酶用于站点融合蛋白的位点特异性裂解，重组融合蛋白含有可进入的凝血酶识别站点，用于去除亲和标签。凝血酶已用于研究马富血小板血浆的活化。
凝血酶用于站点融合蛋白的位点特异性裂解，重组融合蛋白含有可进入的凝血酶识别站点，用于去除亲和标签。
生化/生理作用
选择性裂解纤维蛋白原中 Arg-Gly 键形成纤维蛋白和纤维蛋白肽 A 和 B 的丝氨酸蛋白酶。
单位定义
通过与 NIH 凝血酶参比标准品（批次 K）直接比较，以 NIH 单位表示活性。
外形
从生理盐水柠檬酸钠缓冲液 (pH 6.5) 冻干
分析说明
NIH 试验程序使用 0.2ml 稀释血浆（与生理盐水 1:1）作为底物，0.1根据改进的 Biggs 方法，ml 凝血酶样本（在 1% 缓冲白蛋白溶液中稳定）。仅使用 15-25 秒范围内的凝血时间测定凝血酶浓度。
其他说明
查看更多关于 凝血酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer。
底物
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
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商品
Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.
凝血酶因子IIa是一种内切丝氨酸蛋白酶，可选择性地裂解纤维蛋白原的Arg -Gly键，形成纤维蛋白并释放纤维蛋白肽A和B。
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