所有图片(2)
别名:
BM蓝, Sure Blue TMB, TMB, TMB印迹Plus, TMB底物
线性分子式:
[-C6H2(CH3)2-4-NH2]2
CAS号:
分子量:
240.34
Beilstein:
2808541
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.83
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应用
3,3′,5,5′-四甲基联苯胺已用作竞争法和间接法酶联免疫吸附分析(ELISA)的底物。
3,3′,5,5′-四甲基联苯胺（TMB）是一种二氨基联苯胺的非致癌性取代物（Ames测试阴性）适合在ELISA实验中作为过氧化物酶底物。底物产生可溶性终产物，淡蓝色，分光光度检测在370或620-650nm可以读峰值。TMB反应能被2M H2SO4终止（产生黄色），在450nm处可读。一种高敏感，特异性试剂用于检测血液，血红蛋白实验，过氧化物酶实验。
警示用语：
Warning
危险声明
危险分类
Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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