T4444
3, 3′,5 ,5′-Tetramethylbenzidine
peroxidase substrate, chromogenic, aqueous solution
别名:
TMB
产品名称
3,3′,5,5′-四甲基联苯胺液体底物，超灵敏，用于 ELISA, ready to use solution
质量水平
表单
aqueous solution
运输
wet ice
储存温度
2-8°C
SMILES字符串
Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
InChI
1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChI key
UAIUNKRWKOVEES-UHFFFAOYSA-N
一般描述
本品为包含3,3′,5,5′-四甲基联苯胺（TMB）的弱酸性缓冲液。底物以单组分即用型溶液提供。速率动力学约比传统TMB配方快40％。不推荐用于需要沉淀反应产物的膜或免疫组化应用。
应用
3,3′,5,5′-四甲基联苯胺（TMB）是适用于ELISA程序的过氧化物酶底物。底物产生可溶的浅蓝色最终产物，并且可以在370或655nm下通过分光光度计读取。可以用2M H2SO4停止TMB反应（产生黄色溶液），并在450nm处读取数值。
的3,3′,5,5′-四甲基联苯胺是超灵敏的ELISA液体底物，可用以下应用的作过氧化物酶底物：
- 通过转染子测定合成的透明质酸和HAS2（透明质酸合成酶）表达
- 测定结合的α-溶血素毒素的水平
- 结合IgG成像
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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Behavioural pharmacology, 30(2 and 3-Spec Issue), 260-271 (2019-02-07)
Maternal stress and early life stress affect development. Zebrafish (Danio rerio) are ideally suited to study this, as embryos develop externally into free-feeding larvae. The objective of this study was therefore to assess the effects of increased levels of cortisol
The activity of hyaluronan synthase 2 is regulated by dimerization and ubiquitination
Karousou E, et al.
The Journal of Biological Chemistry, 285(31), 23647-23654 (2010)
E Brandon Strong et al.
Scientific reports, 9(1), 7-7 (2019-01-11)
Microfluidic paper-based analytical devices (microPADs) are emerging as cost-effective and portable platforms for point-of-care assays. A fundamental limitation of microPAD fabrication is the imprecise nature of most methods for patterning paper. The present work demonstrates that paper patterned via wax
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Uchida K, et al.
Blood, 113(11), 2547-2556 (2009)
商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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