应用
该产品的典型用途是使贴壁细胞从培养表面脱落。使细胞从附着面脱落所需的胰蛋白酶浓度主要取决于细胞类型和培养阶段。它可以和内皮细胞培养物一起使用。
生化/生理作用
胰蛋白酶可切割赖氨酸和精氨酸残基的C端肽。 如果酸性残基位于切割位点的两侧，则该反应的水解速度减慢，而如果脯氨酸残基位于切割位点的羧基侧，则不水解。 胰蛋白酶活性的最佳pH为7-9。 胰蛋白酶还可以切割氨基酸合成衍生物的酯键和酰胺键。 将EDTA作为螯合剂添加到胰蛋白酶溶液中，可中和掩盖胰蛋白酶作用肽键的钙镁离子。 去除这些离子可增加酶活性。
DFP、TLCK、APMSF、AEBSEF和抑肽酶等丝氨酸蛋白酶抑制剂可抑制胰蛋白酶。
DFP、TLCK、APMSF、AEBSEF和抑肽酶等丝氨酸蛋白酶抑制剂可抑制胰蛋白酶。
组分
胰蛋白酶由包含223个氨基酸残基的单链多肽组成，通过去除胰蛋白酶原（在Lys-lle肽键处被切割）中的N-末端六肽而产生。氨基酸序列通过6个二硫键交联。这是胰蛋白酶、β-胰蛋白酶的天然形式。β-胰蛋白酶可以自动裂解，在Lys-Ser残基处切割，产生α-胰蛋白酶。胰蛋白酶是丝氨酸蛋白酶家族的成员。
注意
本品应置于 -10 至 -40°C 环境冷冻储存。避免反复冻融。
相关产品
产品编号
说明
价格
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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实验方案
胰蛋白酶常用于从表面进行贴壁细胞的解离。我们可提供一系列不同的胰蛋白酶溶液来满足您的特定细胞系需求。
Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.
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