T4019
胰蛋白酶−琼脂糖
buffered aqueous suspension, from bovine pancreas trypsin
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关于此项目
Biological source:
bovine pancreas (trypsin)
Concentration:
≥15 units/mL (packed gel)
biological source
bovine pancreas (trypsin)
form
buffered aqueous suspension
concentration
≥15 units/mL (packed gel)
extent of labeling
≥15 units per mL (packed gel)
matrix
beaded agarose
storage temp.
2-8°C
Quality Level
General description
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
Application
An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA.
Physical form
Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative
Preparation Note
TPCK-treated
Other Notes
One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
此项目有
Ziad Sahab et al.
Journal of virology, 86(17), 9465-9473 (2012-06-29)
The high-risk human papillomavirus type 16 (HPV-16) E5 protein (16E5) induces tumors in a transgenic mouse model and may contribute to early stages of cervical carcinogenesis. Although high-risk E5 expression is generally thought to be lost during the progression to
Anna Kutschenko et al.
Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiology, 130(6), 1066-1073 (2019-03-16)
Botulinum neurotoxin serotypes A and B (BoNT/A & B) are highly effective medicines to treat hyperactive cholinergic neurons. Due to neutralizing antibody formation, some patients may become non-responders. In these cases, the serotypes BoNT/C-G might become treatment alternatives. BoNT/D is
Fuying Chen et al.
EMBO molecular medicine, 14(5), e14904-e14904 (2022-04-02)
In this report, we discovered a new entity named cataract, alopecia, oral mucosal disorder, and psoriasis-like (CAOP) syndrome in two unrelated and ethnically diverse patients. Furthermore, patient 1 failed to respond to regular treatment. We found that CAOP syndrome was caused
Lucilene Delazari dos Santos et al.
Proteomics, 12(17), 2682-2693 (2012-07-05)
It is well known that the activation of mast cells due to the binding of mastoparan to the G(α) subunit of trimeric G proteins involves exocytosis regulation. However, experimental evidence in the literature indicates that mastoparan can also activate certain
Kevin J Roberts et al.
Scientific reports, 11(1), 19422-19422 (2021-10-02)
Anti-TNFα and anti-IL-23 antibodies are highly effective therapies for Crohn's disease or ulcerative colitis in a proportion of patients. V56B2 is a novel bispecific domain antibody in which a llama-derived IL-23p19-specific domain antibody, humanised and engineered for intestinal protease resistance
相关内容
QC Methods
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