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安全信息

T3824

Sigma-Aldrich

酪氨酸酶 来源于蘑菇

lyophilized powder, ≥1000 unit/mg solid

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别名:
儿茶酚氧化酶, 单酚单加氧酶, 单酚，二羟基苯丙氨酸：氧氧化还原酶, 多酚氧化酶
CAS号:
9002-10-2
EC 号:
1.14.18.1 (BRENDA, IUBMB)
MDL编号:
MFCD00082118
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

fungus (mushroom)

质量水平

200

形式

lyophilized powder

比活

≥1000 unit/mg solid

分子量

119.5 kDa by electrophoresis
 128 kDa by sedimentation velocity diffusion
 133 kDa by LS

应用

diagnostic assay manufacturing

运输

wet ice

储存温度

−20°C

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一般描述

等电点（pI）： 4.7-5
最适pH是6-7

分子量： 128 kDa通过沉降速度扩散；133kDa通过光散射测量，而119.5 kDa通过电泳。

酪氨酸酶是一种含铜氧化酶，对儿茶酚和甲酚都具有活性。 它负责褐变反应。 该酶据报道有两个用于芳族底物的结合位点以及一个用于氧-铜的不同结合位点。 这里的铜很可能是Cu(I)，并包括涉及将其氧化至Cu(II)离子的失活。
酪氨酸酶是一种含铜氧化酶，对儿茶酚和甲酚具有活性。 酪氨酸酶参与酪氨酸向二羟基苯丙氨酸(DOPA)的转化，而DOPA聚合则导致黑色素形成。酪氨酸酶还参与褐变反应。 据称该酶具有两个芳香族底物结合位点和一个独有的氧铜结合位点。

应用

酪氨酸酶活性在一项被开发用于治疗色素沉着过度的替代治疗剂的研究中得到了评估。 酪氨酸酶也用于研究巴基斯坦人群中的眼皮肤白化病的表型。

生化/生理作用

将酪氨酸转化成L-DOPA或酪胺转化成多巴胺

物理属性

等电点（pI）： 4.7-5
最适pH是6-7

分子量： 128 kDa通过沉降速度扩散；133kDa通过光散射测量，而119.5 kDa通过电泳。

单位定义

一个单位 = 在含有 L-酪氨酸的3 mL反应混合液中每分钟产生0.0001的ΔA280，在pH 6.5，25 °C的条件下。

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How can Tyrosinase from mushroom, Product T3824, be dissolved and what is its expected stability in solution?

    Tyrosinase can be dissolved in 50 mM potassium phosphate buffer at 2 mg/mL.  Solutions are expected to retain activity for several days in the refrigerator (2-8°C) and for several weeks in the freezer (-20°C).

  4. The website says Product T3824, Tyrosinase from mushroom, should be stored at -20°C, but I received it at room temperature. Is this correct?

    The product is shipped at ambient temperature and is stable for up to two weeks during shipment.  Once received, it is recommended to store the product at -20°C to insure the quality for long term storage.

  5. I see that the approximate activity of Product T3824, Tyrosinase from mushroom, is  ?1000 unit/mg solid, but how can I find the lot specific activity of this tyrosinase?

    The certificate of analysis for the lot will report the exact tyrosinase activity for a particular batch. The lot number can be found on the label of the bottle, or you can call technical service (800-325-5832) for a sample lot number to view the online certificate of analysis.

  6. I used to order another tyrosinase (Product No. T7755), which has since been discontinued. What is the difference between the replacement product T3824 and the discontinued product T7755?

    The difference between these two products is that T3824 has been stabilized with trehalose, while T7755 was not.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

A T S Jorge et al.
International journal of cosmetic science, 34(5), 435-440 (2012-06-29)
Several treatments for skin whitening are available today, but few of them are completely adequate, especially owing to the carcinogenic potential attributed to classical drugs like hydroquinone, arbutin and kojic acid. To provide an alternative and safer technology for whitening
Shilpi Goenka et al.
Molecules (Basel, Switzerland), 25(16) (2020-08-13)
Previous studies have reported that estrogen hormone promotes melanogenesis while progesterone inhibits it. A selective estrogen receptor modulator (SERM), tamoxifen, has been shown to promote melanogenesis; however, to date, there have been no reports on the effects of a selective
Wangsa T Ismaya et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 67(Pt 5), 575-578 (2011-05-06)
Tyrosinase catalyzes the conversion of tyrosine to dihydroxyphenylalanine quinone, which is the main precursor for the biosynthesis of melanin. The enzyme from Agaricus bisporus, the common button mushroom, was purified and crystallized in two different space groups. Crystals belonging to
Tyrosinase inhibitory effects and inhibition mechanisms of nobiletin and hesperidin from citrus peel crude extracts.
Zhang, et al.
Journal of Enzyme Inhibition and Medicinal Chemistry, 22, 83-90 (2019)
Novel mutations of the tyrosinase (TYR) gene in type I oculocutaneous albinism (OCA1).
R A Spritz et al.
Human mutation, 10(2), 171-174 (1997-01-01)
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