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Merck
CN

T3405

Sigma-Aldrich

3,3′,5,5′-四甲基联苯胺 二盐酸盐

tablet, 1 mg substrate per tablet

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别名:
4,4′-二氨基-3,3′,5,5′-四甲基联苯 二盐酸盐
经验公式(希尔记法):
C16H20N2 · 2HCl
CAS号:
分子量:
313.27
EC 号:
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.83

质量水平

形式

tablet

mp

≥300 °C

储存温度

2-8°C

SMILES字符串

Cl[H].Cl[H].Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H

InChI key

NYNRGZULARUZCC-UHFFFAOYSA-N

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相关类别

应用

3,3′,5,5′-四甲基联苯胺二盐酸已作为底物用于
  • 小鼠血清ELISA检测中的小鼠IgG(γ特异性)辣根过氧化物酶偶联抗体
  • 初始和效应T细胞裂解物的蛋白酪氨酸磷酸酶检测中的小鼠过氧化物酶抗体
  • 糖尿病患者血清样本的氢过氧化物酶法(EH)检测和氧化/抗氧化平衡(PAB)检测

3,3′,5,5′-四甲基联苯胺 (TMB) 是一种适用于酶联免疫吸附分析的过氧化物酶底物。底物产生一种可溶性淡蓝色终产物,该产物可通过分光光度法在 370 或 620-650nm 处读取。可用 2M H2SO4 终止 TMB 反应(变成黄色),并在 450nm 处读取数据。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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商品

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

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