product name
3,3′,5,5′-四甲基联苯胺, ≥98% (TLC)
质量水平
检测方案
≥98% (TLC)
形式
powder
mp
168-171 °C (lit.)
溶解性
ethyl acetate: 50 mg/mL, clear to very slightly hazy
储存温度
2-8°C
SMILES字符串
Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
InChI
1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChI key
UAIUNKRWKOVEES-UHFFFAOYSA-N
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相关类别
应用
3,3′,5,5′-四甲基联苯胺已用于：
- Man-Rodosa-Sharpe（MRS）琼脂平皿的补充剂，在存在O2的条件下将乳杆菌菌落染成蓝色
- 用作ELISA（酶联免疫吸附测定）的底物溶液
- 定量中性粒细胞的髓过氧化物酶活性测定
3,3′,5,5′-四甲基联苯胺（TMB）是一种二氨基联苯胺的非致癌性取代物（Ames测试阴性）适合在ELISA实验中作为过氧化物酶底物。底物产生可溶性终产物，淡蓝色，分光光度检测在370或620-650nm可以读峰值。TMB反应能被2M H2SO4终止（产生黄色），在450nm处可读。一种高敏感，特异性试剂用于检测血液，血红蛋白实验，过氧化物酶实验。
生化/生理作用
3,3′,5,5′-四甲基联苯胺/ TMB可用作色原，加速过氧化物酶反应获取产物的进程。在食品和环境净化程序中，TMB可现场监测亚氯酸基消毒剂的游离氯(FAC)。
包装
无底玻璃瓶。内容物在插入式融锥内。
警示用语：
Warning
危险声明
危险分类
Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
A Felten et al.
Journal of clinical microbiology, 37(3), 729-733 (1999-02-13)
Lactobacilli recovered from the blood, cerebrospinal fluid, respiratory tract, and gut of 20 hospitalized immunocompromised septic patients were analyzed. Biochemical carbohydrate fermentation and total soluble cell protein profiles were used to identify the species. Hydrogen peroxide production was measured. Susceptibility
Ferrotransferrin and antibody against the transferrin receptor as potential vehicles for drug delivery across the mammalian blood-brain barrier into the central nervous system
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The CD4+ T cell-mediated IFN-gamma response to Helicobacter infection is essential for clearance and determines gastric cancer risk
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Journal of immunology (Baltimore, Md. : 1950), 182(11), 7085-7101 (2009)
Lactobacillus casei improves resistance to pneumococcal respiratory infection in malnourished mice
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The Journal of Nutrition, 135(6), 1462-1469 (2005)
Tetramethylbenzidine method for monitoring the free available chlorine and microbicidal activity of chlorite-based sanitizers under organic-matter-rich environments
Yamaoka H, et al.
Letters in Applied Microbiology, 62(1), 47-54 (2016)
商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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