推荐产品
等级
for molecular biology
for molecular biology
质量水平
无菌性
0.2 μm filtered
产品线
BioReagent
形式
solution
浓度
1 M
杂质
DNase, RNase, NICKase and protease, none detected
pH值（酸碱度）
7.35-7.45
SMILES字符串
Cl.NC(CO)(CO)CO
InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key
QKNYBSVHEMOAJP-UHFFFAOYSA-N
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一般描述
Trizma ®盐酸盐溶液是一种有用的生物缓冲液。
应用
采用 Trizma ®盐酸盐与 Trizma-base 制备 pH 7.35 的缓冲液。它被用于制备氚核浸提液。可用于从 嗜热脂肪芽孢杆菌和枯草芽孢杆菌中分离的蛋白酶活性分析。可用作从肠球菌分离株和大肠埃希菌中分离的基因组 DNA 的限制性片段分析的缓冲液 。
所有缓冲液的 pH 值都与温度和浓度相关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。
对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
其他说明
用 pH 可调的生物技术性能认证的 Trizma 碱在 18 兆欧水中制备并经过 0.2μm 过滤器过滤。
法律信息
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Reconstitution and purification of the D-glucose transporter from human erythrocytes.
M Kasahara et al.
The Journal of biological chemistry, 252(20), 7384-7390 (1977-10-25)
El-Refaie Kenawy et al.
Journal of controlled release : official journal of the Controlled Release Society, 81(1-2), 57-64 (2002-05-07)
Electrospun fiber mats are explored as drug delivery vehicles using tetracycline hydrochloride as a model drug. The mats were made either from poly(lactic acid) (PLA), poly(ethylene-co-vinyl acetate) (PEVA), or from a 50:50 blend of the two. The fibers were electrospun
M Fujii et al.
Journal of bacteriology, 154(2), 831-837 (1983-05-01)
The structural gene for a thermostable protease from Bacillus stearothermophilus was cloned in plasmid pTB90. It is expressed in both B. stearothermophilus and Bacillus subtilis. B. stearothermophilus carrying the recombinant plasmid produced about 15-fold more protease (310 U/mg of cell
B E Murray et al.
Journal of clinical microbiology, 28(9), 2059-2063 (1990-09-01)
Epidemiologic evaluation of enterococci has been limited by the lack of a simple and effective method for comparing strains. In this study, we have compared chromosomal restriction endonuclease digestion patterns of 27 isolates of Enterococcus faecalis from three different locations
Pengyi Yang et al.
Cell systems, 8(5), 427-445 (2019-05-13)
Pluripotency is highly dynamic and progresses through a continuum of pluripotent stem cell states. The two states that bookend the pluripotency continuum, naive and primed, are well characterized, but our understanding of the intermediate states and transitions between them remains
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