等级
BioPerformance Certified
for molecular biology
质量水平
形式
solution
浓度
1 M
技术
cell culture | mammalian: suitable
杂质
DNase, RNase, Protease, none detected
bioburden, tested
endotoxin, tested
≤5 ppm Heavy metals (as Pb)
pH值（酸碱度）
7.4
有效pH范围
7.0-9.0
吸光度
≤0.05 at 290 at 40%
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一般描述
一系列 TRIZMA 碱和 TRIZMA HCl 的预混合溶液，为 Tris 缓冲液提供常用的 pH 值。不必混合或调节 pH 值。保证精确度 ± 0.1 个 pH 单位。
应用
Trizma ® 盐酸盐溶液曾用于：
- 制备细胞裂解缓冲液
- 作为洗涤缓冲液和 RNase mix 1 的组分，用于制备 5′-用于 CdS 合成的溶解镉或 S 2- 载微反应器的双末端测序
- 的完整 cDNA
法律信息
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Lab-on-a-micromotor: catalytic Janus particles as mobile microreactors for tailored synthesis of nanoparticles
Pacheco M, et al.
Chemical Science, 9(42), 8056-8064 (2018)
RAMPAGE: Promoter Activity Profiling by Paired-End Sequencing of 5?-Complete cDNAs
Batut P and Gingeras TR
Current Protocols in Molecular Biology, 104(1), 25B-211 (2013)
Determination of Rate of [3H-methyl]-choline Incorporation into Cellular Lipids and Non-lipid Metabolites
Smith T and Phyu S
Bio-protocol, 6(22) (2016)
Matthieu Dos Santos et al.
STAR protocols, 2(3), 100694-100694 (2021-08-13)
Single-nucleus RNA sequencing allows the profiling of gene expression in isolated nuclei. Here, we describe a step-by-step protocol optimized for adult mouse skeletal muscles. This protocol provides two main advantages compared to the widely used single-cell protocol. First, it allows
Kenneth Shatzkes et al.
Scientific reports, 4, 4659-4659 (2014-04-12)
Sample nucleic acid purification can often be rate-limiting for conventional quantitative PCR (qPCR) workflows. We recently developed high-throughput virus microneutralization assays using an endpoint assessment approach based on reverse transcription qPCR (RT-qPCR). The need for cumbersome RNA purification is circumvented
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