一般描述
Plasmid pAR1219 is a pBR322-based vector that expresses T7 RNA Polymerase under control of the IPTG inducible lacUV5 promoter and is intended for use with the TargeTron Gene Knockout System (TA0100).
应用
Many TargeTron system plasmids use the T7 promoter for intron expression. By co-transforming plasmid pAR1219 with the TargeTron pACD4 plasmids, the T7 promoter can be used to express the intron and disrupt chromosomal genes in alternative hosts such as Salmonella typhimurium and Shigella flexneri. Chromosomal gene disruptions in non-DE3 strains of E. coli can also be performed using pAR1219 with the pACD4 intron expression plasmids. TargeTron™ Vector pAR1219 has been used for the overexpression of T7 RNA polymerase in the preparation of S30-T7 lysate. It has also been used to transform MRE600 E. coli strains.
制备说明
To use pAR1219 in conjunction with the pACD4 plasmids, simply co-transform both plasmids and select in a liquid medium containing: 50 mg/ml ampicillin, 25 mg/ml chloramphenicol, and 1% glucose. Glucose is typically included to provide additional suppression of the lac UV5 promoter prior to IPTG-induction.
其他说明
For more information and to view applications data, please visit www.sigma-aldrich.com/targetron.
法律信息
TargeTron is a trademark of InGex, LLC
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
Group II introns as controllable gene targeting vectors for genetic manipulation of bacteria.
Karberg M, et al.
Nature Biotechnology, 19(12), 1162-1162 (2001)
A simple and rapid method for preparing a cell-free bacterial lysate for protein synthesis.
Krinsky N, et al.
PLoS ONE, 11(10), e0165137-e0165137 (2016)
Synthetic Cells Synthesize Therapeutic Proteins inside Tumors.
Krinsky N, et al.
Advanced Helathcare Materials (2017)
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门