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安全信息

T1827

Sigma-Aldrich

Monoclonal Anti-TBP 小鼠抗

clone 58C9, purified from hybridoma cell culture

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别名:
Anti-GTF2D, Anti-GTF2DI, Anti-SCA17, Anti-TATA box binding protein, Anti-TFIID
MDL编号:
MFCD01322691
UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

mouse

质量水平

200

偶联物

unconjugated

抗体形式

purified from hybridoma cell culture

抗体产品类型

primary antibodies

克隆

58C9

分子量

antigen ~42 kDa

种属反应性

Sf9 cell line, human, yeast, Drosophila melanogaster

技术

immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using nuclear extracts of D.Mel cells

同位素/亚型

IgG2b

UniProt登记号

P20226

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

Drosophila melanogaster ... Tbp(37476)
human ... TBP(6908)

一般描述

Monoclonal Anti-TBP (mouse IgG2b isotype) is derived from the hybridoma 58C9 produced by the fusion of mouse myeloma cells and splenocytes from Swiss Webster mice immunized with Drosophila TFIID complex. Transcription factor IID (TFIID) complex contains the TATA-binding protein (TBP) and over a dozen of TBP-associated factors (TAFs).
TBP (TATA-binding protein) is encoded by the gene mapped to human chromosome 6q27. The encoded protein is a key constituent of RNA polymerase II transcription apparatus in eukaryotic cells.

免疫原

Drosophila TFIID complex.

应用

Monoclonal Anti-TBP antibody produced in mouse has been used in:
  • immunoblotting
  • co-immunoprecipitation
  • immunohistochemistry

生化/生理作用

TBP facilitates promoter recognition with the help of the sequence-specific binding of the TATA element found in many promoters.
The TBP (TATA-binding protein) gene encodes a subunit of TFIIIB (transcription factor IIIB) complex and recognizes the TATA box in the DNA. Thus, it plays an important role in RNA polymerase II transcription machinery. TBP is known to induce stress granule formation in hepatitis B viral infection. Mutation in the gene is associated with the development of spinocerebellar ataxia 17 (SCA17).

目标描述

TBP encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of tr

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品

分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Which light chain is used in Product T1827, Monoclonal Anti-TBP antibody, clone 58C9?

    The light chain for Product T1827, Monoclonal Anti-TBP antibody, clone 58C9 is a lambda light chain.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Enhanced Human Decidual Cell-Expressed FKBP51 May Promote Labor-Related Functional Progesterone Withdrawal
Schatz F, et al.
The American Journal of Pathology, 185(9), 2402-2411 (2015)
K Nakamura et al.
Human molecular genetics, 10(14), 1441-1448 (2001-07-13)
Genetic etiologies of at least 20% of autosomal dominant cerebellar ataxias (ADCAs) have yet to be clarified. We identified a novel spinocerebellar ataxia (SCA) form in four Japanese pedigrees which is caused by an abnormal CAG expansion in the TATA-binding
Taspase1 processing alters TFIIA cofactor properties in the regulation of TFIID.
Malecova B
Transcription, 6, 21-32 (2015)
Shanaz A Ghandhi et al.
BMC medical genomics, 3, 31-31 (2010-07-31)
The existence of a radiation bystander effect, in which non-irradiated cells respond to signals from irradiated cells, is well established. To understand early signaling and gene regulation in bystander cells, we used a bio-informatics approach, measuring global gene expression at
Frederick Schatz et al.
The American journal of pathology, 185(9), 2402-2411 (2015-07-25)
Sustained plasma progesterone (P4) levels suggest initiation of human term labor by functional P4 withdrawal, reflecting reduced progesterone receptor (PR) and/or glucocorticoid receptor (GR) expression or activity. The steroid-induced immunophilin cochaperone FKBP51 inhibits PR- and GR-mediated transcription, suggesting a labor-initiating
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