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安全信息

T1827

Sigma-Aldrich

Monoclonal Anti-TBP 小鼠抗

clone 58C9, purified from hybridoma cell culture

别名:

Anti-GTF2D, Anti-GTF2DI, Anti-SCA17, Anti-TATA box binding protein, Anti-TFIID

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About This Item

MDL编号:
MFCD01322691
UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

mouse

质量水平

200

偶联物

unconjugated

抗体形式

purified from hybridoma cell culture

抗体产品类型

primary antibodies

克隆

58C9

分子量

antigen ~42 kDa

种属反应性

Sf9 cell line, human, yeast, Drosophila melanogaster

技术

immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using nuclear extracts of D.Mel cells

同位素/亚型

IgG2b

UniProt登记号

P20226

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

Drosophila melanogaster ... Tbp(37476)
human ... TBP(6908)

相关类别

一般描述

Monoclonal Anti-TBP (mouse IgG2b isotype) is derived from the hybridoma 58C9 produced by the fusion of mouse myeloma cells and splenocytes from Swiss Webster mice immunized with Drosophila TFIID complex. Transcription factor IID (TFIID) complex contains the TATA-binding protein (TBP) and over a dozen of TBP-associated factors (TAFs).
TBP (TATA-binding protein) is encoded by the gene mapped to human chromosome 6q27. The encoded protein is a key constituent of RNA polymerase II transcription apparatus in eukaryotic cells.

免疫原

Drosophila TFIID complex.

应用

Monoclonal Anti-TBP antibody produced in mouse has been used in:
  • immunoblotting
  • co-immunoprecipitation
  • immunohistochemistry

生化/生理作用

TBP facilitates promoter recognition with the help of the sequence-specific binding of the TATA element found in many promoters.
The TBP (TATA-binding protein) gene encodes a subunit of TFIIIB (transcription factor IIIB) complex and recognizes the TATA box in the DNA. Thus, it plays an important role in RNA polymerase II transcription machinery. TBP is known to induce stress granule formation in hepatitis B viral infection. Mutation in the gene is associated with the development of spinocerebellar ataxia 17 (SCA17).

目标描述

TBP encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of tr

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000249550
0000229860
0000249550
0000229860

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Which light chain is used in Product T1827, Monoclonal Anti-TBP antibody, clone 58C9?

    The light chain for Product T1827, Monoclonal Anti-TBP antibody, clone 58C9 is a lambda light chain.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Enhanced Human Decidual Cell-Expressed FKBP51 May Promote Labor-Related Functional Progesterone Withdrawal
Schatz F, et al.
The American Journal of Pathology, 185(9), 2402-2411 (2015)
SCA17, a novel autosomal dominant cerebellar ataxia caused by an expanded polyglutamine in TATA-binding protein.
Nakamura K, et.al.
Human Molecular Genetics, 10, 1441-1448 (2001)
Molecular mechanisms of Bdp1 in TFIIIB assembly and RNA polymerase III transcription initiation.
Gouge J
Nature Communications, 8, 130-130 (2017)
TATA box-binding protein (TBP)-related factor 2 (TRF2), a third member of the TBP family.
Rabenstein MD
Proceedings of the National Academy of Sciences of the USA, 96, 4791-4796 (1999)
Doubly Spliced RNA of Hepatitis B Virus Suppresses Viral Transcription via TATA-Binding Protein and Induces Stress Granule Assembly.
Tsai KN
Journal of Virology, 89, 11406-11419 (2015)
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