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一般描述
台氏液(Tyrode′s salt solution)用于稀释天然来源的蛋白质成分。平衡盐溶液在细胞培养中起多种作用,包括维持细胞内外的渗透压平衡,用作灌流、运输和稀释用液,并供给细胞正常代谢必需的水和大量无机离子。还提供细胞代谢的基本能量,其缓冲能力可使培养基维持在适宜的pH范围内(7.2-7.6)。
应用
酸性台氏液(Tyrode′s Solution)已用于去除卵母细胞/胚胎的透明带。
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Genetics, 200(2), 423-430 (2015-03-31)
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system is an adaptive immune system in bacteria and archaea that has recently been exploited for genome engineering. Mutant mice can be generated in one step through direct delivery of
Molecular biology of the cell, 28(19), 2470-2478 (2017-08-05)
Asymmetric cell divisions depend on the precise placement of the spindle apparatus. In mammalian oocytes, spindles assemble close to the cell's center, but chromosome segregation takes place at the cell periphery where half of the chromosomes are expelled into small
Journal of cellular physiology, 235(10), 7030-7042 (2020-02-06)
Histone deacetylase 6 (HDAC6) participates in mouse oocyte maturation by deacetylating α-tubulin. However, how HDAC6 expression is regulated in oocytes remains unknown. In the present study, we discovered that mouse oocytes had a high level of HDAC6 expression and a
Nature genetics, 51(5), 844-856 (2019-05-02)
The oocyte epigenome plays critical roles in mammalian gametogenesis and embryogenesis. Yet, how it is established remains elusive. Here, we report that histone-lysine N-methyltransferase SETD2, an H3K36me3 methyltransferase, is a crucial regulator of the mouse oocyte epigenome. Deficiency in Setd2
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(7), 2760-2770 (2017-03-16)
During pregnancy, placental trophoblasts at the feto-maternal interface produce a broad repertoire of microRNA (miRNA) species. These species include miRNA from the primate-specific chromosome 19 miRNA cluster (C19MC), which is expressed nearly exclusively in the placenta. Trafficking of these miRNAs
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Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂
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