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等级
BioPerformance Certified
质量水平
形式
crystalline powder
分子量
average Mw 131.4
技术
cell culture | mammalian: suitable
杂质
DNase, RNase, Protease, NICKase, none detected
endotoxin, tested
total aerobic microbial count, tested
≤0.05 A290
≤0.5% water (Karl Fischer)
≤5 ppm Heavy metals (as Pb)
pH值（酸碱度）
8.22 (37 °C, 0.1 M)(lit.)
8.50 (25 °C, 0.1 M)(lit.)
8.5
9.09 (5 °C, 0.1 M)(lit.)
有效pH范围
7.0-9.0
溶解性
water: soluble, clear
应用
diagnostic assay manufacturing
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应用
Open the foil pouch, dissolve contents in 900 mL of water; add additional water to a final volume of 1 liter to make a 0.1 M solution at a temperature of 25 °C. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.
Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.
If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.
If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
该产品经细胞培养测试。它还经过了低内毒素水平检测并分析了是否已去除核酸酶和蛋白酶。
包装
简易锡箔袋包装
其他说明
This product is a pre-mixed combination of Trizma base and Trizma HCl in convenient foil pouches that can be used to prepare a common pH value for Tris buffer.
某些pH电极不能使用Tris缓冲液提供准确的结果。
制备说明
Trizma溶液可高压灭菌。
法律信息
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
G Travis Tabor et al.
Molecular and cellular neurosciences, 98, 121-130 (2019-06-19)
Kv4.2 voltage-gated K+ channel subunits, the primary source of the somatodendritic A-type K+ current in CA1 pyramidal neurons of the hippocampus, play important roles in regulating dendritic excitability and plasticity. To better study the trafficking and subcellular distribution of Kv4.2
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