生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
WH-3, monoclonal
分子量
antigen 55 kDa
包含
15 mM sodium azide
种属反应性
human, monkey, rabbit, rat
技术
immunohistochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using rabbit pineal gland extract
同位素/亚型
IgG3
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... TPH1(7166) , TPH2(121278)
rat ... Tph1(24848) , Tph2(317675)
一般描述
特异性
免疫原
应用
- 酶联免疫吸附测定(ELISA)
- 免疫组织化学
- 免疫印迹
- 免疫细胞化学
生化/生理作用
免责声明
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WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Can Product T0678, Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse, be used to stain mouse brain sections?
This antibody has been used successfully on mouse brain slices in an immunohistochemical application. The IHC results from an experiment employing T0678 at 1:500 in mouse brain slice staining clearly showed the dorsal nuclear raphe (which are the serotonergic neurons).
Does Product T0678, Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse, cross-react with tyrosine or phenylalanine hydroxylases?
This antibody, unlike some other antibodies to tryptophan hydroxylases (WH) in the market, does not cross react with tyrosine hydroxylase (TH) or with phenylalanine hydroxylase (PH). The epitope recognized by the antibody is not located in the Pterin Binding Region, which is homologous among PH, TH and WH. The specificity to WH is confirmed by the single band obtained at the 55KD region, while TH has a higher MW (60-68KD), depending on the submit) and PH has a lower MW. It has also been confirmed by collaborating experts. See Journal of Neuroscience Methods, 114(2), 205-212 (2002).
Do you have an immunohistochemistry protocol for use with Product T0678, Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse?
Free floating sections (4% paraformaldehyde fixation)1. Incubate 30 minutes at room temperature in 0.3% Triton X-100, 5% normal serum.2. Incubate with the antibody diluted 1:10,000 in PBS, 0.3% Triton X-100, 3.5% normal serum and 0.05% BSA for 36-48 hours.3. Rinse 3 times for 5 minutes each in PBS.4. Add the biotinylated anti-mouse IgG3 diluted in PBS containing 0.3% Triton X-100 and 3.5% normal serum for 1 hour at room temperature.5. Rinse 3 times for 5 minutes each in PBS.6. Process with Avidin Biotin-HRP.7. Complete the reaction with a 2-10 minute incubation in PBS containing 0.05% DAB and 0.015% hydrogen peroxide (H2O2).8. Mount the sections on gel coated slides and air dry.
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